Recovery of cyanophycin granule polypeptide from activated sludge: carbon source dependence and aggregation-induced luminescence characteristics

IF 6.1 2区 环境科学与生态学 Q2 ENGINEERING, ENVIRONMENTAL Frontiers of Environmental Science & Engineering Pub Date : 2023-09-13 DOI:10.1007/s11783-024-1776-7
Kui Zou, Hongyuan Liu, Bo Feng, Taiping Qing, Peng Zhang
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Abstract

In the sewage treatment process, facilitating the conversion of pollutants into value-added resources holds great potential for reducing the amount of greenhouse gas emissions and promoting economic circulation. Cyanophycin granule polypeptide (CGP), a recently discovered high value-added biopolymer present in activated sludge, has provided new avenues for the recovery of resources. However, the mechanisms that regulate CGP synthesis and the characteristics of this biopolymer in activated sludge remain unclear thus far. This study investigated the synthesis of CGP, polyhydroxyalkanoates (PHA), and alginate-like exopolysaccharides (ALE) in various microbial aggregates under different carbon sources feeding conditions. Our results showed that the CGP yields was superior that of PHA and ALE when subjected to identical carbon source feeding conditions. Furthermore, biofilm was more conducive to CGP accumulation than floc sludge. Compared with glucose and methanol, sodium acetate significantly enriched the CGP synthetase-encoding gene (cphAabundance = ~17419), resulting in the highest CGP yield (average 107.1 mg/g MLSS) in both biofilm and floc sludge. This study is the first to reported the characteristic fluorescence of CGP (Ex/Em = ~360/450 nm) caused by the aggregated luminescence of arginine on the side chains. Overall, this study highlights the potential application of CGP as a fluorescent material and offers insights into CGP recovery from activated sludge in wastewater treatment plants.

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从活性污泥中回收藻青素颗粒多肽:碳源依赖性和聚集致发光特性
在污水处理过程中,促进污染物转化为增值资源,对于减少温室气体排放量,促进经济循环具有很大的潜力。青藻素颗粒多肽(CGP)是近年来在活性污泥中发现的一种高附加值生物聚合物,为资源回收提供了新的途径。然而,迄今为止,调节CGP合成的机制和活性污泥中这种生物聚合物的特性仍不清楚。本研究研究了不同碳源饲喂条件下不同微生物聚集体中CGP、聚羟基烷酸酯(PHA)和海藻酸酯样胞外多糖(ALE)的合成。结果表明,在相同碳源加料条件下,CGP的产率优于PHA和ALE。此外,生物膜比絮凝污泥更有利于CGP的积累。与葡萄糖和甲醇相比,乙酸钠显著富集CGP合成酶编码基因(cphAabundance = ~17419),导致生物膜和絮凝污泥中CGP产量最高(平均107.1 mg/g MLSS)。本研究首次报道了由侧链上精氨酸聚集发光引起的CGP特征性荧光(Ex/Em = ~360/450 nm)。总的来说,本研究突出了CGP作为荧光材料的潜在应用,并为废水处理厂活性污泥中CGP的回收提供了见解。
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来源期刊
Frontiers of Environmental Science & Engineering
Frontiers of Environmental Science & Engineering ENGINEERING, ENVIRONMENTAL-ENVIRONMENTAL SCIENCES
CiteScore
10.90
自引率
12.50%
发文量
988
审稿时长
6.1 months
期刊介绍: Frontiers of Environmental Science & Engineering (FESE) is an international journal for researchers interested in a wide range of environmental disciplines. The journal''s aim is to advance and disseminate knowledge in all main branches of environmental science & engineering. The journal emphasizes papers in developing fields, as well as papers showing the interaction between environmental disciplines and other disciplines. FESE is a bi-monthly journal. Its peer-reviewed contents consist of a broad blend of reviews, research papers, policy analyses, short communications, and opinions. Nonscheduled “special issue” and "hot topic", including a review article followed by a couple of related research articles, are organized to publish novel contributions and breaking results on all aspects of environmental field.
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