Aspergillus niger based lipase-tween 80 aggregates as interfacial activated biocatalyst for biodiesel production: Optimization using response surface methodology

IF 1.3 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY Main Group Chemistry Pub Date : 2022-04-22 DOI:10.3233/mgc-220010
Bahar Ghasemzadeh, Amir Abbas Matin, Mostafa Ebadi, Biuck Habibi
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Abstract

Biodiesel is a renewable energy source, which is produced through transesterification reactions. Despite great attention to develop enzymatic biodiesel production, there are serious obstacles to the industrial development of it such as its cost and slow reaction rate. Along with disadvantages, there are several advantages for enzymatic biodiesel production. Higher purity of fuel and glycerol is known as the most important achievement of enzymatic process. In this study, performance of four different fungi for lipase production was investigated and Aspergillus niger was selected as enzyme source. Lipase production were optimized using experimental design and the optimized factors were determined as pH 5, temperature 30 °C, Potato Dextrose Broth (PDB) 3 % w/v, olive oil 1.50 % v/v, with maximum lipase activity of 42.8±0.51 U/mg. In order to interfacial activation of the lipase, effect of surfactants was studied. Therefore, surfactant-enzyme aggregates were used as biocatalyst for transesterification reaction. Effects of factors on biodiesel yield were studied too. The yield was 96.41±1.20 % at the optimized conditions (methanol/oil molar ratio (5.50:1), enzyme concentration 19 % v/ w, Tween 80 concentration 19 mg L– 1, temperature 40 °C and reaction time 46 h).
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以黑曲霉为基础的脂肪酶-吐温80聚集体作为生物柴油生产的界面活化生物催化剂:响应面法优化
生物柴油是一种可再生能源,它是通过酯交换反应生产的。酶法生产生物柴油备受关注,但存在成本高、反应速度慢等严重阻碍其产业化发展。除了缺点之外,酶促生物柴油生产也有一些优点。高纯度的燃料和甘油被认为是酶促过程最重要的成就。在本研究中,研究了四种不同真菌生产脂肪酶的性能,并选择黑曲霉作为酶源。采用实验设计对脂肪酶的生产进行优化,确定最优条件为pH 5,温度30℃,马铃薯葡萄糖肉汤(PDB) 3% w/v,橄榄油1.50% v/v,最大脂肪酶活性为42.8±0.51 U/mg。为了对脂肪酶进行界面活化,研究了表面活性剂的作用。因此,表面活性剂-酶聚合体被用作酯交换反应的生物催化剂。研究了影响生物柴油产率的因素。优化条件为:甲醇/油摩尔比(5.50:1),酶浓度19% v/ w, Tween 80浓度19 mg L-1,温度40℃,反应时间46 h,产率为96.41±1.20%。
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来源期刊
Main Group Chemistry
Main Group Chemistry 化学-化学综合
CiteScore
2.00
自引率
26.70%
发文量
65
审稿时长
>12 weeks
期刊介绍: Main Group Chemistry is intended to be a primary resource for all chemistry, engineering, biological, and materials researchers in both academia and in industry with an interest in the elements from the groups 1, 2, 12–18, lanthanides and actinides. The journal is committed to maintaining a high standard for its publications. This will be ensured by a rigorous peer-review process with most articles being reviewed by at least one editorial board member. Additionally, all manuscripts will be proofread and corrected by a dedicated copy editor located at the University of Kentucky.
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