Aspergillus niger based lipase-tween 80 aggregates as interfacial activated biocatalyst for biodiesel production: Optimization using response surface methodology
Bahar Ghasemzadeh, Amir Abbas Matin, Mostafa Ebadi, Biuck Habibi
{"title":"Aspergillus niger based lipase-tween 80 aggregates as interfacial activated biocatalyst for biodiesel production: Optimization using response surface methodology","authors":"Bahar Ghasemzadeh, Amir Abbas Matin, Mostafa Ebadi, Biuck Habibi","doi":"10.3233/mgc-220010","DOIUrl":null,"url":null,"abstract":"Biodiesel is a renewable energy source, which is produced through transesterification reactions. Despite great attention to develop enzymatic biodiesel production, there are serious obstacles to the industrial development of it such as its cost and slow reaction rate. Along with disadvantages, there are several advantages for enzymatic biodiesel production. Higher purity of fuel and glycerol is known as the most important achievement of enzymatic process. In this study, performance of four different fungi for lipase production was investigated and Aspergillus niger was selected as enzyme source. Lipase production were optimized using experimental design and the optimized factors were determined as pH 5, temperature 30 °C, Potato Dextrose Broth (PDB) 3 % w/v, olive oil 1.50 % v/v, with maximum lipase activity of 42.8±0.51 U/mg. In order to interfacial activation of the lipase, effect of surfactants was studied. Therefore, surfactant-enzyme aggregates were used as biocatalyst for transesterification reaction. Effects of factors on biodiesel yield were studied too. The yield was 96.41±1.20 % at the optimized conditions (methanol/oil molar ratio (5.50:1), enzyme concentration 19 % v/ w, Tween 80 concentration 19 mg L–\n1, temperature 40 °C and reaction time 46 h).","PeriodicalId":18027,"journal":{"name":"Main Group Chemistry","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Main Group Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3233/mgc-220010","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Biodiesel is a renewable energy source, which is produced through transesterification reactions. Despite great attention to develop enzymatic biodiesel production, there are serious obstacles to the industrial development of it such as its cost and slow reaction rate. Along with disadvantages, there are several advantages for enzymatic biodiesel production. Higher purity of fuel and glycerol is known as the most important achievement of enzymatic process. In this study, performance of four different fungi for lipase production was investigated and Aspergillus niger was selected as enzyme source. Lipase production were optimized using experimental design and the optimized factors were determined as pH 5, temperature 30 °C, Potato Dextrose Broth (PDB) 3 % w/v, olive oil 1.50 % v/v, with maximum lipase activity of 42.8±0.51 U/mg. In order to interfacial activation of the lipase, effect of surfactants was studied. Therefore, surfactant-enzyme aggregates were used as biocatalyst for transesterification reaction. Effects of factors on biodiesel yield were studied too. The yield was 96.41±1.20 % at the optimized conditions (methanol/oil molar ratio (5.50:1), enzyme concentration 19 % v/ w, Tween 80 concentration 19 mg L–
1, temperature 40 °C and reaction time 46 h).
期刊介绍:
Main Group Chemistry is intended to be a primary resource for all chemistry, engineering, biological, and materials researchers in both academia and in industry with an interest in the elements from the groups 1, 2, 12–18, lanthanides and actinides. The journal is committed to maintaining a high standard for its publications. This will be ensured by a rigorous peer-review process with most articles being reviewed by at least one editorial board member. Additionally, all manuscripts will be proofread and corrected by a dedicated copy editor located at the University of Kentucky.