No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan

IF 2.7 3区医学 Q3 VIROLOGY Retrovirology Pub Date : 2022-05-18 DOI:10.1186/s12977-022-00592-6

Yamanaka, Meripet Polat, Saito, Susumu, Hara, Yukiko, Matsuura, Ryosuke, Takeshima, Shin-nosuke, Hosomichi, Kazuyoshi, Matsumoto, Yasunobu, Furuta, Rika A., Takei, Masami, Aida, Yoko

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引用次数: 6

Abstract

The potential risk and association of bovine leukemia virus (BLV) with human remains controversial as it has been reported to be both positive and negative in human breast cancer and blood samples. Therefore, establishing the presence of BLV in comprehensive human clinical samples in different geographical locations is essential. In this study, we examined the presence of BLV proviral DNA in human blood and breast cancer tissue specimens from Japan. PCR analysis of BLV provirus in 97 Japanese human blood samples and 23 breast cancer tissues showed negative result for all samples tested using long-fragment PCR and highly-sensitive short-fragment PCR amplification. No IgG and IgM antibodies were detected in any of the 97 human serum samples using BLV gp51 and p24 indirect ELISA test. Western blot analysis also showed negative result for IgG and IgM antibodies in all tested human serum samples. Our results indicate that Japanese human specimens including 97 human blood, 23 breast cancer tissues, and 97 serum samples were negative for BLV.

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在日本人类标本中未发现牛白血病病毒前病毒DNA和抗体的证据

牛白血病病毒(BLV)与人类的潜在风险和相关性仍然存在争议，因为据报道，它在人类乳腺癌和血液样本中均呈阳性和阴性。因此，在不同地理位置的综合人类临床样本中确定BLV的存在至关重要。在这项研究中，我们检测了人类血液和日本乳腺癌组织标本中BLV前病毒DNA的存在。采用长片段PCR和高灵敏度短片段PCR扩增技术对97份日本人血液样本和23份乳腺癌组织的BLV病毒进行PCR分析，所有样本均呈阴性。采用BLV gp51和p24间接ELISA法检测97份人血清，均未检出IgG和IgM抗体。Western blot分析显示，所有人血清IgG和IgM抗体均为阴性。结果表明，日本人血液样本97份，乳腺癌组织样本23份，血清样本97份，BLV阴性。

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来源期刊

Retrovirology 医学-病毒学

CiteScore

5.80

自引率

3.00%

发文量

审稿时长

>0 weeks

期刊介绍： Retrovirology is an open access, online journal that publishes stringently peer-reviewed, high-impact articles on host-pathogen interactions, fundamental mechanisms of replication, immune defenses, animal models, and clinical science relating to retroviruses. Retroviruses are pleiotropically found in animals. Well-described examples include avian, murine and primate retroviruses. Two human retroviruses are especially important pathogens. These are the human immunodeficiency virus, HIV, and the human T-cell leukemia virus, HTLV. HIV causes AIDS while HTLV-1 is the etiological agent for adult T-cell leukemia and HTLV-1-associated myelopathy/tropical spastic paraparesis. Retrovirology aims to cover comprehensively all aspects of human and animal retrovirus research.