Enzymes analysis, degradation kinetics, response surface optimization and heavy metal tolerance of the biodegradation of malachite green by Stenotrophomonas koreensis

IF 1.9 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Bioremediation Journal Pub Date : 2022-11-12 DOI:10.1080/10889868.2022.2143472

Shreya Biswas, Tuhin Kahali, Anwesha Mukherjee, Debasmita Chakraborty, Chayan Guha, Tathagata Adhikary, Pratik Das, Nandan Kumar Jana, Suvendu Manna, Piyali Basak

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Abstract

Stenotrophomonas koreensis, was isolated from a textile effluent and employed to biologically degrade 98.8% of the recalcitrant toxic dye malachite green in 4 h in the absence of any supplements or media. Chromatographic and Spectroscopic analysis confirmed the degradation of the dye. According to Response Surface Methodology, the optimum conditions for biodegradation were pH 6, inoculum size 6 mL (initial concentration Log₁₀CFU/mL = 6.2), and temperature 45 °C. More than 98% decolorization was achieved within 2.5 h of the addition of soymeal extract or peptone. One of the most promising features of this strain is that even in the presence of heavy metals, S. koreensis actively degraded the dye. The bacteria biodegraded malachite green following the first-order reaction kinetics. LC-MS analysis of the degradation product yielded several intermediates like Michler’s ketone (m/z 269), 4-N,N-dimethylaminophenol (m/z 137), Benzophenone (m/z 182), N,N-dimethylaniline (m/z 121), 4-(N-methylamino)-benzophenone (m/z 211), 4-aminobenzaldehyde (m/z 121), anionic canonical form of 4-aminobenzaldehyde (m/z 120), 4-(N,N-dimethylamino)benzoic acid (m/z 165), 4-(N-methylamino) benzoic acid (m/z 152). The enzymes responsible for the malachite green degradation were tyrosinase, Malachite Green reductase, and NADH-DCIP reductase. The present work is the first to report the degradation of malachite green by S. koreensis.

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韩国窄养单胞菌降解孔雀石绿的酶分析、降解动力学、响应面优化及重金属耐受性

摘要从某纺织废水中分离得到韩国窄养单胞菌，在不添加任何培养基的情况下，在4 h内对难降解毒性染料孔雀石绿的98.8%进行了生物降解。色谱和光谱分析证实了染料的降解。根据响应面法，最佳生物降解条件为pH 6、接种量6 mL(初始浓度Log10CFU/mL = 6.2)、温度45℃。在添加豆粕提取物或蛋白胨2.5 h内脱色率达到98%以上。该菌株最有希望的特征之一是，即使在重金属存在的情况下，韩国红藻也能积极地降解染料。细菌按一级反应动力学降解孔雀石绿。降解产物的LC-MS分析得到了几种中间体:米歇尔酮(m/z 269)， 4-N,N-二甲氨基苯酚(m/z 137)，二苯甲酮(m/z 182)， N,N-二甲苯胺(m/z 121)， 4-(N-甲氨基)-二苯甲酮(m/z 211)， 4-氨基苯甲醛(m/z 121)，阴离子典型形式的4-氨基苯甲醛(m/z 120)， 4-(N,N-二甲氨基)苯甲酸(m/z 165)， 4-(N-甲氨基)苯甲酸(m/z 152)。降解孔雀石绿的酶有酪氨酸酶、孔雀石绿还原酶和NADH-DCIP还原酶。本文首次报道了韩国松对孔雀石绿的降解作用。

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来源期刊

Bioremediation Journal ENVIRONMENTAL SCIENCES-

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

9 months

期刊介绍： Bioremediation Journal is a peer-reviewed quarterly that publishes current, original laboratory and field research in bioremediation, the use of biological and supporting physical treatments to treat contaminated soil and groundwater. The journal rapidly disseminates new information on emerging and maturing bioremediation technologies and integrates scientific research and engineering practices. The authors, editors, and readers are scientists, field engineers, site remediation managers, and regulatory experts from the academic, industrial, and government sectors worldwide. High-quality, original articles make up the primary content. Other contributions are technical notes, short communications, and occasional invited review articles.