Straightforward thin-layer chromatography–densitometric method for the determination of phyllanthin in Phyllanthus niruri from different phytogeographical zones

IF 1.1 4区 化学 Q4 CHEMISTRY, ANALYTICAL Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-11-20 DOI:10.1007/s00764-023-00257-w
Kartini Kartini, Alfi Syahr Wijayati, Nikmatul Ikhrom Eka Jayani, Finna Setiawan, Ryanto Budiono
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Abstract

Phyllanthus niruri contains various lignan compounds, whose concentrations vary depending on several factors. This study was intended to determine the phyllanthin content of P. niruri obtained from various locations in Indonesia by using thin-layer chromatography (TLC)‒densitometry to evaluate the effect of geographical factors on their quality. The TLC system comprised silica gel 60 F254 for the stationary phase, toluene‒ethyl acetate‒formic acid (15:10.5:1.5, V/V) for the mobile phase, and documentation under ultraviolet (UV) 254 nm light without chemical reagents. This developed method meets the specificity requirement, as marked by the identical UV spectrum between the phyllanthin sample and the standard (λmax = 279 and 230 nm). Further, it shows good linearity for phyllanthin concentrations in the range of 2.36‒11.8 µg/band (r = 0.9924), with LOD 0.532 µg/band and LOQ 1.612 µg/band. It also has good intraday and interday precision, as indicated by RSD of 8.87–9.43 and 6.94%, respectively. Eight of the 15 analyzed samples (collected from Batu, Blitar, Kediri, Nganjuk, Jember, Mojokerto, Banyuwangi, and Surabaya) contained only a trace amount of phyllanthin. In contrast, the other seven had varying levels of phyllanthin (1.376‒4.130 mg/g dried herbs). Using the Tawangmangu sample as the reference, these seven samples can be grouped into two: significantly lower phyllanthin contents (Tulungagung) and very significantly lower phyllanthin contents (Lumajang, Bangkalan, Pasuruan, Sidoarjo, and Gresik). It can be concluded that TLC‒densitometry designed in this research is a straightforward method that, at the same time, meets the validation parameters. Therefore, it can be repeated to analyze phyllanthin in P. niruri of different phytogeographical origins.

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直接薄层色谱-密度法测定不同植物区带余甘子中余甘素的含量
余甘子含有多种木脂素化合物,其浓度取决于几个因素。本研究采用薄层色谱-密度测定法测定印度尼西亚不同产地黑檀中叶黄素的含量,以评价地理因素对其质量的影响。TLC体系以硅胶60 F254为固定相,甲苯-乙酸乙酯-甲酸(15:10. 5:15 .5,V/V)为流动相,在紫外(UV) 254 nm光下无化学试剂记录。该方法与标准品紫外光谱一致(λmax = 279 nm和230 nm),满足特异性要求。在2.36 ~ 11.8µg/波段范围内呈良好的线性关系(r = 0.9924),检出限为0.532µg/波段,检出限为1.612µg/波段。该方法具有较好的日内、日间精度,RSD分别为8.87 ~ 9.43和6.94%。15个分析样本中有8个(收集自巴图、布利塔、克迪里、恩甘竹、Jember、Mojokerto、Banyuwangi和泗水)仅含有微量叶黄素。相比之下,其他7个品种含有不同水平的叶黄素(1.376 ~ 4.130 mg/g)。以Tawangmangu样品为参照,这7个样品可分为两类:叶黄素含量极低的(Tulungagung)和含量极低的(Lumajang、Bangkalan、Pasuruan、Sidoarjo和Gresik)。由此可见,本研究设计的薄层色谱密度测定法是一种简便易行的方法,同时满足验证参数。因此,可以重复分析不同植物地理来源的叶黄素。
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来源期刊
CiteScore
2.20
自引率
18.80%
发文量
66
审稿时长
>12 weeks
期刊介绍: JPC - Journal of Planar Chromatography - Modern TLC is an international journal devoted exclusively to the publication of research papers on analytical and preparative planar chromatography. The journal covers all fields of planar chromatography, on all kinds of stationary phase (paper, layer, gel) and with various modes of migration of the mobile phase (capillary action or forced flow).
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