{"title":"Bio-Synthesized Silver Nanoparticles Using Cannabis Sativa Seed Extracts and Its Anticancer Effects","authors":"Arife Kübra Yontar, Sinem Çevik","doi":"10.1007/s11468-023-02142-y","DOIUrl":null,"url":null,"abstract":"<div><p>Silver nanoparticles bio-synthesized with <i>Cannabis sativa</i> extracts have low energy consumption and raw material usage, high antibacterial effect, easy production conditions, and low toxicity. With these advantages, it can be a natural alternative to drug delivery systems used in cancer treatments. Therefore, the effects of bio-synthesized nanosilver on A549 lung cancer cells were investigated. The synthesized AgNPs were first confirmed in terms of color changes, absorbance in UV–Vis spectrophotometry, morphological appearance in scanning electron microscopy (SEM) analysis, and EDS. Visible blackish color development was noticed during the 1-h production period, with a rising peak recorded at 420 nm and an average size of 43 nm; highly triangular and quasi-spherical AgNPs were observed in the SEM images. The Fourier transform infrared spectroscopy (FTIR) confirmed that phenolic groups from plant extracts formed new chemical bonds with AgNPs. AgNPs were determined to be 280% more effective against <i>E. coli</i> and 85% against <i>S. aureus</i> bacteria, one of the ampicillin antibiotics. In the MTT test, different concentrations of AgNPs capped with <i>Cannabis sativa</i> extracts showed a 100% inhibitory effect at 375 μg mL<sup>−1</sup> against the A549 liver cancer cell line after 48 h of exposure. In this study, tests were performed only on A549 lung cancer cells. It revealed that AgNPs synthesized from extracts of <i>Cannabis sativa</i> seeds could be used as a potential candidate to develop nanomedicines for the treatment of lung cancer in the near future. It has been determined that treatment opportunities can be increased with studies on cells of different cancer diseases.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":736,"journal":{"name":"Plasmonics","volume":"19 4","pages":"2031 - 2043"},"PeriodicalIF":3.3000,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plasmonics","FirstCategoryId":"101","ListUrlMain":"https://link.springer.com/article/10.1007/s11468-023-02142-y","RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
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Abstract
Silver nanoparticles bio-synthesized with Cannabis sativa extracts have low energy consumption and raw material usage, high antibacterial effect, easy production conditions, and low toxicity. With these advantages, it can be a natural alternative to drug delivery systems used in cancer treatments. Therefore, the effects of bio-synthesized nanosilver on A549 lung cancer cells were investigated. The synthesized AgNPs were first confirmed in terms of color changes, absorbance in UV–Vis spectrophotometry, morphological appearance in scanning electron microscopy (SEM) analysis, and EDS. Visible blackish color development was noticed during the 1-h production period, with a rising peak recorded at 420 nm and an average size of 43 nm; highly triangular and quasi-spherical AgNPs were observed in the SEM images. The Fourier transform infrared spectroscopy (FTIR) confirmed that phenolic groups from plant extracts formed new chemical bonds with AgNPs. AgNPs were determined to be 280% more effective against E. coli and 85% against S. aureus bacteria, one of the ampicillin antibiotics. In the MTT test, different concentrations of AgNPs capped with Cannabis sativa extracts showed a 100% inhibitory effect at 375 μg mL−1 against the A549 liver cancer cell line after 48 h of exposure. In this study, tests were performed only on A549 lung cancer cells. It revealed that AgNPs synthesized from extracts of Cannabis sativa seeds could be used as a potential candidate to develop nanomedicines for the treatment of lung cancer in the near future. It has been determined that treatment opportunities can be increased with studies on cells of different cancer diseases.
期刊介绍：
Plasmonics is an international forum for the publication of peer-reviewed leading-edge original articles that both advance and report our knowledge base and practice of the interactions of free-metal electrons, Plasmons.
Topics covered include notable advances in the theory, Physics, and applications of surface plasmons in metals, to the rapidly emerging areas of nanotechnology, biophotonics, sensing, biochemistry and medicine. Topics, including the theory, synthesis and optical properties of noble metal nanostructures, patterned surfaces or materials, continuous or grated surfaces, devices, or wires for their multifarious applications are particularly welcome. Typical applications might include but are not limited to, surface enhanced spectroscopic properties, such as Raman scattering or fluorescence, as well developments in techniques such as surface plasmon resonance and near-field scanning optical microscopy.
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