Acetylcholinesterase and choline acetyltransferase activity in some hypothalamic nuclei under immobilization stress in rats.

Endocrinologia experimentalis Pub Date : 1989-03-01
M Fatranská, A Kiss, Z Oprsalová, R Kvetnanský
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引用次数: 0

Abstract

Rats were subjected to acute immobilization (IMO) for 1 or 2 h and killed by decapitation. The hypothalamic supraoptic and paraventricular nuclei were isolated by dissection technique and the activity of acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) was assayed. A period of 1 or 2 h of IMO resulted in a significant increase of AChE activity only in the supraoptic nucleus, while no change of ChAT activity occurred. Acute IMO of 1 or 2 h duration resulted in an increase of AChE activity in supraoptic nucleus.

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固定应激下大鼠下丘脑部分核的乙酰胆碱酯酶和胆碱乙酰转移酶活性。
大鼠急性固定化(IMO) 1 ~ 2 h,斩首处死。采用解剖法分离下丘脑视上核和室旁核,测定其乙酰胆碱酯酶(AChE)和胆碱乙酰转移酶(ChAT)活性。IMO作用1或2 h时,AChE活性仅在视上核显著升高,ChAT活性未发生变化。急性IMO持续1或2 h可导致视上核AChE活性升高。
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