Long Noncoding RNAs MEG3, TUG1, and hsa-miR-21-3p Are Potential Diagnostic Biomarkers for Coronary Artery Disease

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Pub Date : 2023-12-07 DOI:10.1134/s0026893324010126

M. Abdelgawad, H. Y. Abdallah, A. Fareed, A. E. Ahmed

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Abstract

Peripheral blood biomarkers are of particular importance to diagnose certain diseases including coronary artery disease (CAD) due to their non-invasiveness. Investigating the expression of noncoding RNAs (ncRNAs) paves the way to early disease diagnosis, prognosis, and treatment. Consequently, in this research, we aimed to investigate a panel of ncRNAs as potential biomarkers in patients with coronary artery disease. Two different groups have been designed (control and CAD). All participants were subjected to interviews and clinical examinations. Peripheral blood samples were collected, and plasma was extracted. At the same time, target ncRNAs have been selected based on literature review and bioinformatic analysis, and later they underwent investigation using quantitative real-time PCR. The selected panel encompassed the long non-coding RNAs (lncRNAs) MEG3, TUG1, and SRA1, and one related microRNA (miRNA): hsa-miR-21-3p. We observed statistically significant upregulation in MEG3, TUG1, and hsa-miR21-3p in CAD patients compared to control participants (p-value < 0.01). Nevertheless, SRA1 exhibited downregulation with no statistical significance (p-value > 0.05). All ncRNAs under study displayed a significantly strong correlation with disease incidence, age, and smoking. Network construction revealed a strong relationship between MEG3 and TUG1. ROC analysis indicated high potentiality for hsa-miR-21-3p to be a promising biomarker for CAD. Moreover, MEG3 and TUG1 displayed distinguished diagnostic discrimination but less than hsa-miR-21-3p, all of them exhibited strong statistical significance differences between CAD and control groups. Conclusively, this research pinpointed that MEG3, TUG1, and hsa-miR-21-3p are potential biomarkers of CAD incidence and diagnosis.

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长非编码 RNA MEG3、TUG1 和 hsa-miR-21-3p 是冠状动脉疾病的潜在诊断生物标记物

摘要外周血生物标志物因其非侵入性而对诊断包括冠状动脉疾病（CAD）在内的某些疾病具有特别重要的意义。研究非编码 RNA（ncRNA）的表达为疾病的早期诊断、预后和治疗铺平了道路。因此，在这项研究中，我们旨在研究冠心病患者中作为潜在生物标志物的一组 ncRNA。我们设计了两个不同的小组（对照组和冠心病患者组）。所有参与者都接受了访谈和临床检查。收集外周血样本并提取血浆。同时，根据文献综述和生物信息学分析筛选出目标 ncRNA，随后使用实时定量 PCR 对其进行研究。所选的ncRNA包括长非编码RNA（lncRNA）MEG3、TUG1和SRA1，以及一种相关的microRNA（miRNA）：hsa-miR-21-3p。与对照组相比，我们观察到 CAD 患者的 MEG3、TUG1 和 hsa-miR21-3p 有明显的统计学上的上调（p 值为 0.01）。然而，SRA1 出现了下调，但无统计学意义（p 值为 0.05）。研究中的所有 ncRNA 都与疾病发病率、年龄和吸烟有明显的密切关系。网络构建显示 MEG3 和 TUG1 之间存在密切关系。ROC分析表明，hsa-miR-21-3p极有可能成为CAD的生物标志物。此外，MEG3 和 TUG1 的诊断鉴别力也很高，但低于 hsa-miR-21-3p，它们在 CAD 组和对照组之间都有很强的统计学差异。最终，这项研究指出，MEG3、TUG1 和 hsa-miR-21-3p 是潜在的 CAD 发病率和诊断的生物标志物。

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来源期刊

Molecular Biology 生物-生化与分子生物学

CiteScore

1.30

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.