The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Pub Date : 2023-12-07 DOI:10.1134/s0026893323060110

E. G. Novoselova, O. V. Glushkova, M. O. Khrenov, S. M. Lunin, M. G. Sharapov, R. G. Goncharov, E. K. Mubarakshina, T. V. Novoselova, S. B. Parfenyuk

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Abstract—The aim of this work was to study the effects of thymosin-1 alpha (Tα1) on the anti-inflammatory response of RAW 264.7 macrophages cultured in the presence of lipopolysaccharide (LPS) from the walls of gram-negative bacteria. As well, we evaluated production of pro-inflammatory cytokines and the activity of the NF-κB and SAPK/JNK signaling pathways. In addition, the level of expression of a number of genes that regulate cell apoptosis, as well as the activity of receptors involved in the pro-inflammatory response, was determined. First, the addition of Tα1 normalized the level of cytokine production to varying degrees, with a particularly noticeable effect on IL-1β and IL-6. Second, the addition of Tα1 normalized the activity of the NF-κB and SAPK/JNK signaling cascades and the expression of the Tlr4 gene. Third, Tα1 significantly reduced p53 and the activity of the P53 gene, which is a marker of cell apoptosis. Fourth, it was shown that the increase in Ar-1 gene expression under the influence of LPS was significantly reduced using Tα1. Thus, it was found that the presence of Tα1 in the RAW 264.7 cell culture medium significantly reduced the level of the pro-inflammatory response of cells.

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胸腺激素胸腺素-1α可降低内毒素诱导的生264.7细胞的促炎反应

摘要--这项工作的目的是研究胸腺肽-1α（Tα1）对在革兰氏阴性菌壁脂多糖（LPS）存在下培养的 RAW 264.7 巨噬细胞抗炎反应的影响。我们还评估了促炎细胞因子的产生以及 NF-κB 和 SAPK/JNK 信号通路的活性。此外，我们还测定了一些调节细胞凋亡的基因的表达水平，以及参与促炎反应的受体的活性。首先，Tα1 的加入在不同程度上使细胞因子的产生水平趋于正常，对 IL-1β 和 IL-6 的影响尤为明显。其次，添加 Tα1 能使 NF-κB 和 SAPK/JNK 信号级联的活性以及 Tlr4 基因的表达正常化。第三，Tα1 能明显降低 p53 和作为细胞凋亡标志的 P53 基因的活性。第四，研究表明，Tα1 能明显降低 LPS 影响下 Ar-1 基因表达的增加。因此，研究发现 Tα1 在 RAW 264.7 细胞培养基中的存在明显降低了细胞的促炎反应水平。

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来源期刊

Molecular Biology 生物-生化与分子生物学

CiteScore

1.30

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.