O. I. Kravchuk, A. D. Finoshin, K. V. Mikhailov, R. H. Ziganshin, K. I. Adameyko, N. G. Gornostaev, A. I. Zhurakovskaya, V. S. Mikhailov, E. I. Shagimardanova, Yu. V. Lyupina
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引用次数: 0
Abstract
δ-Aminolevulinic acid dehydratase (ALAD) is a key enzyme of the cytoplasmic heme biosynthesis pathway. The primary structure of the ALAD gene, the multimeric structure of the ALAD/hemB protein, and ALAD expression during the annual reproductive cycle were studied in the cold-water marine sponge Halisarca dujardinii. The results implicated the GATA-1 transcription factor and DNA methylation in regulating ALAD expression. Re-aggregation of sponge cells was accompanied by a decrease in ALAD expression and a change in the cell content of an active ALAD/hemB form. Further study of heme biosynthesis and the role of ALAD/hemB in morphogenesis of basal animals may provide new opportunities for treating pathologies in higher animals.
摘要δ-氨基乙酰脱水酶（ALAD）是细胞质血红素生物合成途径中的一个关键酶。研究了冷水海海绵Halisarca dujardinii中ALAD基因的一级结构、ALAD/hemB蛋白的多聚体结构以及ALAD在年生殖周期中的表达。研究结果表明，GATA-1 转录因子和 DNA 甲基化参与了 ALAD 表达的调控。海绵细胞的重新聚集伴随着 ALAD 表达的减少和活性 ALAD/hemB 形式细胞含量的变化。进一步研究血红素的生物合成以及 ALAD/hemB 在基础动物形态发生中的作用，可能会为治疗高等动物的病症提供新的机会。
期刊介绍：
Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.
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