In silico epitope prediction of Borrelia burgdorferi sensu lato antigens for the detection of specific antibodies

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of immunological methods Pub Date : 2023-12-07 DOI:10.1016/j.jim.2023.113596

Weronika Grąźlewska, Karolina Sołowińska, Lucyna Holec-Gąsior

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Abstract

Despite many years of research, serodiagnosis of Lyme disease still faces many obstacles. Difficulties arise mainly due to the low degree of amino acid sequence conservation of the most immunogenic antigens among B. burgdorferi s.l. genospecies, as well as differences in protein production depending on the environment in which the spirochete is located. Mapping B-cell epitopes located on antigens allows for a better understanding of antibody-pathogen interactions which is essential for the development of new and more effective diagnostic tools. In this study, in silico B-cell epitope mapping was performed to determine the theoretical diagnostic potential of selected B. burgdorferi s.l. proteins (BB0108, BB0126, BB0298, BB0689, BB0323, FliL, PstS, SecD, EF-Tu). Bioinformatics software predicted 35 conserved linear and 31 conformational epitopes with the degree of identity among B. burgdorferi s.l. of at least 85%, which may prove to be useful in the development of a new tool for the diagnosis of Lyme disease.

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为检测特异性抗体而对包柔氏病原抗原的表位进行硅学预测

尽管经过多年研究，莱姆病的血清诊断仍面临许多障碍。出现这些困难的主要原因是，在 B. burgdorferi s.l. 基因种间，最具免疫原性的抗原的氨基酸序列保存程度较低，而且由于螺旋体所处的环境不同，蛋白质的产生也存在差异。绘制抗原上的 B 细胞表位图可以更好地了解抗体与病原体之间的相互作用，这对于开发新的、更有效的诊断工具至关重要。在这项研究中，为了确定所选 B. burgdorferi s.l. 蛋白质（BB0108、BB0126、BB0298、BB0689、BB0323、FliL、PstS、SecD、EF-Tu）的理论诊断潜力，对其进行了 B 细胞表位硅图绘制。生物信息学软件预测了 35 个保守的线性表位和 30 个构象表位，这些表位在 B. burgdorferi s.l. 中的相同度至少为 85%，这些表位可能被证明有助于开发诊断莱姆病的新工具。

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来源期刊

Journal of immunological methods 医学-免疫学

CiteScore

4.10

自引率

0.00%

发文量

120

审稿时长

3 months

期刊介绍： The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens. (2) Purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies. (5) Localizing antigens and/or antibodies in tissues and cells. (6) Detecting, and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Documenting cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. (12) Molecular techniques for studying immune cells and their receptors. (13) Imaging of the immune system. (14) Methods for production or their fragments in eukaryotic and prokaryotic cells. In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods. In general these manuscripts are by solicitation however anyone interested in submitting a review can contact the Reviews Editor and provide an outline of the proposed review.