Co-printing of micro/nanostructures integrated with preconcentration to enhance protein detection

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Microfluidics and Nanofluidics Pub Date : 2023-12-09 DOI:10.1007/s10404-023-02699-4
Yi-Jung Lu, Han-Yun Hsieh, Wen-Fai Yang, Kuang-Chong Wu, Hidetoshi Tahara, Pei-Kuen Wei, Horn-Jiunn Sheen, Yu-Jui Fan
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Abstract

This paper reports a fabrication method that can make microstructures such as microfluidic channels and nanostructures to generate surface plasmon resonance (SPR) signals in one-step using hot embossing. We first made a micro/nanostructural mold on a silicon substrate through sequential e-beam lithography, reactive ion etching (RIE), photolithography, and inductively coupled plasma RIE. The fabricated mold and cyclo-olefin polymer (COP) film were pressed between two flat, heated metal bases under optimal conditions, and the micro/nanostructures were complementarily transferred to the COP film. After depositing a thin aluminum film onto the nanostructure, the device was completed by patterning Nafion that crossed two channels and a nearby nanostructure, and by bonding the COP film to a flat polydimethylsiloxane (PDMS) substrate with holes punched for the inlets and outlets. SPR signals of the nanostructures of the microfluidic channel were calibrated using glycerol solutions of different percentages, and a wavelength sensitivity of 393 nm/refractive index unit was found for the Al-based nanoslit SPR sensing chip. To detect macromolecules, we first modified bovine serum albumin (BSA) onto the surface of the SPR chip and then allowed different concentrations of anti-BSA samples to flow into the device. A calibration curve for detecting anti-BSA was constructed, and anti-BSA detection levels with and without preconcentration were compared.

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集成预浓缩功能的微/纳米结构共打印技术可提高蛋白质检测能力
本文报告了一种制造方法,该方法可以利用热压印技术一步制成微流体通道等微结构和纳米结构,从而产生表面等离子体共振(SPR)信号。我们首先通过电子束光刻、活性离子刻蚀(RIE)、光刻和电感耦合等离子体 RIE 在硅基底上制作了微/纳米结构模具。在最佳条件下,将制作好的模具和环烯烃聚合物(COP)薄膜压在两个平整、加热的金属基座之间,然后将微/纳米结构互补地转移到 COP 薄膜上。在纳米结构上沉积一层薄铝膜后,对穿过两个通道和附近纳米结构的 Nafion 进行图案化,并将 COP 薄膜粘合到平面聚二甲基硅氧烷(PDMS)基底上,在入口和出口处打孔,从而完成了该装置。使用不同比例的甘油溶液对微流体通道纳米结构的 SPR 信号进行了校准,发现铝基纳米光 SPR 传感芯片的波长灵敏度为 393 nm/折射率单位。为了检测大分子,我们首先在 SPR 芯片表面修饰了牛血清白蛋白(BSA),然后让不同浓度的抗 BSA 样品流入该装置。我们构建了检测抗 BSA 的校准曲线,并比较了有无预浓缩的抗 BSA 检测水平。
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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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