The Anti-cancer Effect of Two Extract Fractions from the Hemiscorpius lepturus Scorpion Venom

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2023-12-10 DOI:10.1134/s0003683823060145

Z. Setayesh-Mehr, A. Asoodeh, L. V. Ghasemi

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Abstract

In this study, the fraction of the Hemiscorpius lepturus scorpion venom was subjected. The two bioactive fractions (F2 and F4) were separated and collected by RP-HPLC. Next, the cytotoxicity of F2 and F4 against human red blood cells and MCF-7 cell line was investigated. The expression levels of Bax, Bcl-2, and p53 genes in cancer cells treated with fractions were determined. In order to evaluate the effects of the two extract fractions, breast cancer was induced in BALB-c mice developed by the injection of MCF-7 cells. Following the intraperitoneal injection of F2 and F4 into mice, the levels of IFN-γ and IL-4 were measured using ELISA. The effects of fractions on the survival of MCF-7 cancer cells were significant compared to normal cells (p < 0.001), while they had no significant hemolysis activity against red blood cells (p > 0.05). The results showed that the expression of Bax and p53 was increased in MCF-7 cells treated with fractions compared to control cells without treatment, while the expression of Bcl-2 was decreased. The results of the in vivo analysis showed that the level of IFN-γ was increased, whereas the level of IL-4 was significantly reduced in mice treated with F2 and F4 at the concentrations of 5 and 10 mg/kg compared to control animals (p < 0.0001). Based on the results, two extract fractions of H. lepturus scorpion venom exert anti-cancer properties, and they are able to modulate the immune system and induce apoptosis in cancer cells.

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Hemiscorpius lepturus 蝎毒的两种提取物的抗癌效果

摘要本研究以 Hemiscorpius lepturus 蝎子毒液为研究对象。采用 RP-HPLC 方法分离并收集了两种生物活性馏分（F2 和 F4）。接着，研究了 F2 和 F4 对人红细胞和 MCF-7 细胞株的细胞毒性。此外，还测定了经馏分处理的癌细胞中 Bax、Bcl-2 和 p53 基因的表达水平。为了评估两种提取物馏分的作用，在 BALB-c 小鼠体内注射 MCF-7 细胞诱发乳腺癌。小鼠腹腔注射 F2 和 F4 后，使用 ELISA 法测定 IFN-γ 和 IL-4 的水平。与正常细胞相比，馏分对 MCF-7 癌细胞存活率的影响显著（p < 0.001），而它们对红细胞没有明显的溶血活性（p > 0.05）。结果表明，与未处理的对照细胞相比，用馏分处理的 MCF-7 细胞中 Bax 和 p53 的表达量增加，而 Bcl-2 的表达量减少。体内分析结果表明，与对照组相比，使用浓度为 5 毫克/千克和 10 毫克/千克的 F2 和 F4 处理的小鼠体内 IFN-γ 的水平升高，而 IL-4 的水平则显著降低（p < 0.0001）。以上结果表明，两种蝎毒提取物具有抗癌特性，它们能够调节免疫系统并诱导癌细胞凋亡。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.