Analysis of bacteria, inflammation, and exudation in epidermal suction blister wounds reveals dynamic changes during wound healing

Sigrid Lundgren, Ganna Petruk, Karl Wallblom, José FP Cardoso, Ann-Charlotte Strömdahl, Fredrik Forsberg, Congyu Luo, Bo Nilson, Erik Hartman, Jane Fisher, Manoj Puthia, Karim Saleh, Artur Schmidtchen
{"title":"Analysis of bacteria, inflammation, and exudation in epidermal suction blister wounds reveals dynamic changes during wound healing","authors":"Sigrid Lundgren, Ganna Petruk, Karl Wallblom, José FP Cardoso, Ann-Charlotte Strömdahl, Fredrik Forsberg, Congyu Luo, Bo Nilson, Erik Hartman, Jane Fisher, Manoj Puthia, Karim Saleh, Artur Schmidtchen","doi":"10.1101/2023.12.07.23299659","DOIUrl":null,"url":null,"abstract":"The skin microbiome undergoes dynamic changes during different phases of wound healing, however the role of bacteria in the wound healing process remains poorly described. In this study, we aimed to determine how wound bacteria develop over time in epidermal wounds, and how they interact with inflammatory processes during wound healing. To this end, we analyzed wound fluid and swab samples collected from epidermal suction blister wounds in healthy volunteers. We found that bacterial numbers, measured in swabs and dressing fluid, increased rapidly after wounding and stabilized by day 8. The composition of bacterial species identified by MALDI-TOF mass spectrometry differed between wounds, but generally consisted primarily of commensal bacteria and remained largely stable over time. Inflammation and neutrophil activity, measured by quantification of cytokines and neutrophil proteins in dressing fluid, peaked on day 5. Exudation, measured by quantification of protein content in dressings, also peaked at this time and strongly correlated with cytokine and neutrophil protein levels. Inflammation, neutrophil activity, and exudation were not correlated with bacterial counts at any time, indicating that in normally healing wounds, these processes are primarily driven by the host and are independent of colonizing bacteria. Our analysis provides a comprehensive understanding of epidermal wound healing dynamics in the host and the role of the microbiome in healthy wound healing.","PeriodicalId":501385,"journal":{"name":"medRxiv - Dermatology","volume":"3 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"medRxiv - Dermatology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1101/2023.12.07.23299659","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The skin microbiome undergoes dynamic changes during different phases of wound healing, however the role of bacteria in the wound healing process remains poorly described. In this study, we aimed to determine how wound bacteria develop over time in epidermal wounds, and how they interact with inflammatory processes during wound healing. To this end, we analyzed wound fluid and swab samples collected from epidermal suction blister wounds in healthy volunteers. We found that bacterial numbers, measured in swabs and dressing fluid, increased rapidly after wounding and stabilized by day 8. The composition of bacterial species identified by MALDI-TOF mass spectrometry differed between wounds, but generally consisted primarily of commensal bacteria and remained largely stable over time. Inflammation and neutrophil activity, measured by quantification of cytokines and neutrophil proteins in dressing fluid, peaked on day 5. Exudation, measured by quantification of protein content in dressings, also peaked at this time and strongly correlated with cytokine and neutrophil protein levels. Inflammation, neutrophil activity, and exudation were not correlated with bacterial counts at any time, indicating that in normally healing wounds, these processes are primarily driven by the host and are independent of colonizing bacteria. Our analysis provides a comprehensive understanding of epidermal wound healing dynamics in the host and the role of the microbiome in healthy wound healing.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
对表皮吸疱伤口中的细菌、炎症和渗出进行分析,揭示伤口愈合过程中的动态变化
在伤口愈合的不同阶段,皮肤微生物群会发生动态变化,但细菌在伤口愈合过程中的作用仍鲜为人知。在这项研究中,我们旨在确定伤口细菌在表皮伤口中是如何随着时间的推移而发展的,以及它们在伤口愈合过程中是如何与炎症过程相互作用的。为此,我们分析了从健康志愿者表皮吸疱伤口采集的伤口液和拭子样本。我们发现,拭子和敷料液中的细菌数量在伤口愈合后迅速增加,并在第 8 天稳定下来。通过 MALDI-TOF 质谱鉴定出的细菌种类组成在不同伤口之间存在差异,但一般主要由共生细菌组成,并且随着时间的推移基本保持稳定。通过对敷料液中的细胞因子和中性粒细胞蛋白进行定量测定,炎症和中性粒细胞活性在第 5 天达到高峰。通过量化敷料中的蛋白质含量测量的渗出量也在此时达到峰值,并与细胞因子和中性粒细胞蛋白质水平密切相关。炎症、中性粒细胞活性和渗出在任何时候都与细菌数量无关,这表明在正常愈合的伤口中,这些过程主要由宿主驱动,与定植细菌无关。我们的分析提供了对宿主表皮伤口愈合动态以及微生物组在健康伤口愈合中的作用的全面了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
A Two-step Bayesian Mendelian Randomization Study on Cholecystitis and Dermatitis Risk of Major Adverse Cardiovascular Events following Nicotinamide Exposure: Cohort Study Methodological issues in visible LED therapy dermatological research and reporting Leveraging Machine Learning & Mobile Application Technology for Vitiligo Management: A Proof-of-Concept Nationwide melanoma registry databases in real-world settings: a scoping review protocol
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1