Extracellular activity of proteases from Yarrowia lipolytica IPS21 as a function of the carbon and nitrogen source

IF 0.7 4区 工程技术 Q3 MATERIALS SCIENCE, TEXTILES Fibres & Textiles in Eastern Europe Pub Date : 2023-12-06 DOI:10.2478/ftee-2023-0046
Dorota Wieczorek, Katarzyna Miśkiewicz, D. Gendaszewska, Paulina Pipiak, M. Lasoń-Rydel, Katarzyna Sieczyńska, K. Ławińska
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Abstract

Abstract The yeast strain Yarrowia lipolytica IPS 21 was tested for its ability to produce the protease enzyme on analytically pure carbon sources as well as on waste carbon sources. It was confirmed that the yeast Y. lipolytica IPS21 can have a higher proteolytic activity in the presence of waste carbon sources in chrome-tanned leather shavings (CTLS) than on yeast extract alone. This is confirmed by the high concentration of amino acids in samples with CTLS, suggesting increased degradation of CTLS by Y. lipolytica or secretion of proteases into the medium. It was also confirmed that metals accumulate mainly in the biomass and not in the supernatant. The biomass was also found to contain high levels of Ca, K and P, which are essential for plant growth. These results show that Y. lipolytica strain IPS21 can be used for the production of extracellular alkaline proteases and for the degradation of protein waste.
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脂溶性亚罗菌 IPS21 蛋白酶的胞外活性与碳源和氮源的关系
摘要研究了酵母解脂耶氏菌IPS 21在纯碳源和废碳源上产生蛋白酶的能力。结果表明,在铬鞣皮屑(CTLS)废碳源存在的情况下,酵母脂解酵母IPS21具有更高的蛋白水解活性。CTLS样品中氨基酸浓度高证实了这一点,表明溶脂Y.菌对CTLS的降解或向培养基中分泌蛋白酶增加。金属主要在生物量中积累,而不是在上清液中积累。生物量还被发现含有高水平的钙、钾和磷,这是植物生长所必需的。这些结果表明,该菌株IPS21可用于生产细胞外碱性蛋白酶和降解蛋白质废物。
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来源期刊
Fibres & Textiles in Eastern Europe
Fibres & Textiles in Eastern Europe 工程技术-材料科学:纺织
CiteScore
1.60
自引率
11.10%
发文量
12
审稿时长
13.5 months
期刊介绍: FIBRES & TEXTILES in Eastern Europe is a peer reviewed bimonthly scientific journal devoted to current problems of fibre, textile and fibrous products’ science as well as general economic problems of textile industry worldwide. The content of the journal is available online as free open access. FIBRES & TEXTILES in Eastern Europe constitutes a forum for the exchange of information and the establishment of mutual contact for cooperation between scientific centres, as well as between science and industry.
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