Integrated analysis of differentially expressed genes implicated in ovarian cancer progression

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-12-01 DOI:10.4274/tjod.galenos.2023.65072

Bahriye Gür, Nurhan Külcü Sarıkaya, Deniz Sünnetçi Akkoyunlu

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Abstract

Objective: Ovarian cancer (OC) is a common gynecological malignancy associated with high morbidity and generally poor prognosis despite treatment. The aim of this study was to understand the influence of gene expression differences and pathways in OC development and progression. Materials and Methods: One hundred and thirty-three OC samples and 34 normal ovarian tissues were included in the study from the Gene Expression Omnibus database. GeneSpring Software was used to obtain differentially expressed genes (DEGs) in all stages comparing tumor and normaltissue. DEGs were analyzed using the DAVID interface for Kyoto Encyclopedia of Genes and Genomes pathway analysis. Most most connected genes were selected as hub genes for each stage using the STRING application in Cytoscape software. Results: DEGs were found to be associated with cell cycle and herpes simplex virus infection pathways. A total of 19 genes (ACTB, AKT1, ALB, CTNNB1, EGFR, EP300, ESR1, FN1, GAPDH, HSPA4, IL6, JUN, MYC, PTEN, RPS27A, SRC, TNF, TP53 and UBC) were identified as hub genes. Among the hub genes, the TP53 gene was found to have the highest level of connections in all stages. EGFR, RPS27A, and AKT1 were found to have high numbers of connections in stages II, III, and IV, respectively. Conclusion: The results of the current study may provide new insights into OC pathogenesis and suggest potential prognostic and therapeutic targets.

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综合分析与卵巢癌进展有关的差异表达基因

目的:卵巢癌(OC)是一种常见的妇科恶性肿瘤，发病率高，治疗后预后差。本研究的目的是了解基因表达差异和途径在卵巢癌发生和进展中的影响。材料与方法:从Gene Expression Omnibus数据库中选取133例卵巢癌样本和34例正常卵巢组织作为研究对象。使用genesspring软件获得肿瘤和正常组织各阶段的差异表达基因(DEGs)。使用京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes)通路分析的DAVID接口分析deg。利用Cytoscape软件中的STRING应用程序选择连接最多的基因作为每个阶段的枢纽基因。结果:发现deg与细胞周期和单纯疱疹病毒感染途径有关。共有19个基因(ACTB、AKT1、ALB、CTNNB1、EGFR、EP300、ESR1、FN1、GAPDH、HSPA4、IL6、JUN、MYC、PTEN、RPS27A、SRC、TNF、TP53和UBC)被鉴定为枢纽基因。在枢纽基因中，TP53基因在所有阶段的连接水平最高。EGFR、RPS27A和AKT1分别在II期、III期和IV期有大量的连接。结论:本研究结果可能为卵巢癌的发病机制提供新的见解，并提示潜在的预后和治疗靶点。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.