Activities and concentration of alpha-1 antitrypsin and cystatin C in serum from patients with house dust mite asthma

IF 1.6 Q3 ALLERGY Asia Pacific Allergy Pub Date : 2023-12-01 DOI:10.5415/apallergy.0000000000000124

Mailani Dwi Hidayati, F. C. Iswanti, Samsuridjal Djauzi, Sukamto Koesnoe, Mohamad Sadikin

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Abstract

Background: The proteolytic activities of house dust mite (HDM) allergens are involved in the pathogenesis of asthma by cleaving T-junction protein complexes, increasing the permeability of airway epithelial cells, and enabling the allergens to reach the interstitial tissue. The human body contains natural protease inhibitors such as alpha-1 antitrypsin (AAT) with antiserine protease activity and cystatin C with anticysteine protease activity. Objective: This study aimed to investigate the behavior of serum AAT and cystatin C levels in patients with HDM-allergic asthma. Methods: Ten individuals with HDM-allergic asthma and 10 healthy volunteers participated in a cross-sectional study. The serum AAT and cystatin C inhibitory activities were measured using enzymatic assays. ELISA was used to determine the serum AAT and cystatin C concentrations. Results: Serum AAT inhibitory activity (P = 0.445; P > 0.05), AAT concentration (P = 0.290; P > 0.05), and cystatin C concentration (P = 0.419; P > 0.05) did not significantly differ between the patient and control groups. However, serum cystatin C inhibitory activity in the asthmatic patient group was significantly higher than in the healthy subjects (P = 0.001; P < 0.05). There was no correlation between AAT inhibitory activity and AAT concentration or between cystatin C inhibitory activity and cystatin C concentration. Conclusion: These findings suggest that serum cystatin C activity is involved in asthma pathogenesis. Additional research is required to address this issue.

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屋尘螨哮喘患者血清中α-1抗胰蛋白酶和胱抑素C的活性和浓度

背景:屋尘螨(HDM)变应原的蛋白水解活性通过裂解t连接蛋白复合物，增加气道上皮细胞的通透性，使变应原到达间质组织参与哮喘的发病。人体内含有天然的蛋白酶抑制剂，如具有抗丝氨酸蛋白酶活性的α -1抗胰蛋白酶(AAT)和具有抗半胱氨酸蛋白酶活性的胱抑素C。目的:探讨hdm变应性哮喘患者血清AAT和胱抑素C水平的变化。方法:10例hdm变应性哮喘患者和10名健康志愿者进行横断面研究。酶促法测定血清AAT和胱抑素C抑制活性。ELISA法测定血清AAT和胱抑素C浓度。结果:血清AAT抑制活性(P = 0.445;P > 0.05)， AAT浓度(P = 0.290;P > 0.05)，胱抑素C浓度(P = 0.419;P > 0.05)，两组间差异无统计学意义。而哮喘患者组血清胱抑素C抑制活性显著高于健康组(P = 0.001;P < 0.05)。AAT抑制活性与AAT浓度、胱抑素C抑制活性与胱抑素C浓度无相关性。结论:血清胱抑素C活性参与哮喘发病过程。需要进一步的研究来解决这个问题。

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来源期刊

Asia Pacific Allergy ALLERGY-

CiteScore

2.50

自引率

5.90%

发文量

期刊介绍： Asia Pacific Allergy (AP Allergy) is the official journal of the Asia Pacific Association of Allergy, Asthma and Clinical Immunology (APAAACI). Although the primary aim of the journal is to promote communication between Asia Pacific scientists who are interested in allergy, asthma, and clinical immunology including immunodeficiency, the journal is intended to be available worldwide. To enable scientists and clinicians from emerging societies appreciate the scope and intent of the journal, early issues will contain more educational review material. For better communication and understanding, it will include rational concepts related to the diagnosis and management of asthma and other immunological conditions. Over time, the journal will increase the number of original research papers to become the foremost citation journal for allergy and clinical immunology information of the Asia Pacific in the future.