Features of Induction of In Vitro Osteodifferentiation of Adipose-Derived Mesenchymal Stem Cells

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2023-12-14 DOI:10.1134/s0003683823080021

N. M. Aleksandrova, A. M. Aimaletdinov, A. G. Malanyeva, M. A. Tambovskii, A. A. Rizvanov, E. Yu. Zakirova

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Abstract

The features of osteogenic differentiation of mesenchymal stem cells (MSCs) of dogs, native and genetically modified, have been compared when cultivated in different media. It was shown that calcium deposition into the extracellular matrix occurred within 21 day of cultivation of MSCs on a commercial induction medium. The use of α-MEM-based medium, which contains osteoinducing components, also caused osteogenic differentiation of MSCs, but calcium deposition was less active. Gene modification of canine MSCs with a plasmid carrying the gene for canine (Canis familiaris) bone morphogenetic protein 2 gene (BMP2) stimulated the release of alkaline phosphatase by cells, but not calcium deposition. Consequently, the formation of osteocytes from MSCs depends not only on endogenous, but also exogenous factors, which include dexamethasone in combination with ascorbic acid and β-glycerophosphate, and full osteogenic differentiation of MSCs in vitro requires many osteoinductive factors.

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诱导脂肪间充质干细胞体外骨分化的特点

比较了犬间充质干细胞(MSCs)在不同培养基中培养时的成骨分化特征。结果表明，在商业诱导培养基上培养MSCs后21天内，钙沉积到细胞外基质中。使用含有成骨成分的α- mems基质也能引起MSCs的成骨分化，但钙沉积活性较低。携带犬骨形态发生蛋白2基因(BMP2)的质粒对犬间充质干细胞进行基因修饰，可促进细胞释放碱性磷酸酶，但不能促进钙沉积。因此，MSCs成骨细胞的形成不仅取决于内源性因素，也取决于外源性因素，其中包括地塞米松与抗坏血酸和β-甘油磷酸酯的联合作用，MSCs在体外的完全成骨分化需要许多成骨诱导因子。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.