Genetic difference between two Schistosoma japonicum isolates with contrasting cercarial shedding patterns revealed by whole genome sequencing

IF 2.3 2区医学 Q2 PARASITOLOGY Parasite Pub Date : 2023-12-12 DOI:10.1051/parasite/2023061

Hui-Ying Sun, Jie-Ying Zhang, Han-Xiang Zhang, Qing Xu, Da-Bing Lu

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Abstract

Schistosoma japonicum is one of the major infectious agents of human schistosomiasis, mainly endemic in China and the Philippines. We have previously reported the finding of two schistosome isolates, each with a different cercarial emergence pattern adapted to their different hosts. However, there are currently no whole-genome sequencing studies to investigate the underlining genetics of the adaptive traits. We sampled schistosomes in 2013 and 2020 from a hilly area Shitai (ST) and a marshland area Hexian (HX) of Anhui, China. Ten to 15 male or female adult worms from each site/year were sent for whole genome sequencing. Genetics were analyzed, and selection signals along genomes were detected. Gene enrichment analysis was performed for the genome regions under selection. The results revealed considerable genetic differentiation between the two isolates. The genome “windows” affected by natural selection were fewer in ST (64 windows containing 78 genes) than in HX (318 windows containing 276 genes). Twelve significantly enriched genes were identified in ST, but none in HX. These genes were mainly related to specific DNA binding and intercellular signaling transduction. Some functional region changes identified along the genome of the hilly schistosome may be related to its unique late afternoon cercarial emergence.

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通过全基因组测序发现两种日本血吸虫分离株的遗传差异，它们的蛛网膜脱落模式截然不同

日本血吸虫(Schistosoma japonicum)是人类血吸虫病的主要传染源之一，主要在中国和菲律宾流行。我们以前报道过发现两种分离的血吸虫，每一种都有适应其不同宿主的不同子宫颈出现模式。然而，目前还没有全基因组测序研究来调查适应性状的潜在遗传学。我们于2013年和2020年在中国安徽石台丘陵地区和河县沼泽地区采集血吸虫样本。每年从每个站点抽取10 ~ 15只雌雄成虫进行全基因组测序。遗传学分析，并检测到沿基因组的选择信号。对选择的基因组区域进行基因富集分析。结果显示两个分离株之间存在相当大的遗传分化。受自然选择影响的基因组“窗口”在ST(64个窗口，包含78个基因)比HX(318个窗口，包含276个基因)少。在ST中发现了12个显著富集的基因，而在HX中没有。这些基因主要与特异性DNA结合和细胞间信号转导有关。沿丘陵血吸虫基因组鉴定的一些功能区变化可能与其独特的下午晚些时候的子宫颈出现有关。

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来源期刊

Parasite 医学-寄生虫学

CiteScore

5.50

自引率

6.90%

发文量

审稿时长

3 months

期刊介绍： Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.