Assessment of photosynthetic capacity of two blackberry cultivars subjected to salt stress by the JIP fluorescence test

IF 1.5 4区农林科学 Q3 PLANT SCIENCES Journal of Berry Research Pub Date : 2023-12-15 DOI:10.3233/jbr-230026

Ines Mihaljević, Marija Viljevac Vuletić, Vesna Tomaš, Zvonimir Zdunić, Dominik Vuković, Krunoslav Dugalić

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Abstract

BACKGROUND: Salt stress has recently emerged as one of the most significant abiotic stresses with negative impact on crop production. Photosynthesis is a vital biochemical process in plants, very sensitive to environmental constraints, including salt stress. OBJECTIVE: The aim of this study was toevaluate the impact of salt stress on photosystem II in the leaves of blackberry cultivars (Rubus fructicosus L.). METHODS: Two blackberry cultivars (Thornfree and Boysen) were exposed to salt stress (20, 40, and 60 mM NaCl) for 28 days in in vitro culture. The photosynthetic activity was evaluated by probing chlorophyll a fluorescence (JIP test) and photosynthetic pigments. RESULTS: The maximum quantum yield of PSII (Fv/Fm), performance indexes (PIABS and PItotal), and photosynthetic driving force (DFABS) were greatly affected by salt stress in the leaves of Boysen cultivar. Lower photosynthetic efficiency was confirmed by the increased values of absorption flux per RC (ABS/RC), trapping flux per PSII RC (TR0/RC), dissipation flux per RC (DI0/RC), decreased values of chlorophyll and carotenoid contents, disruption in the interaction between the light-harvesting complex and the core proteins of PSII, as well as disruption of the oxygen-evolving complex. CONCLUSIONS: The provided informations suggest that the photosynthetic apparatus of Thornfree cultivar was found to be more tolerant to salt stress compared to Boysen.

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通过 JIP 荧光测试评估两种受到盐胁迫的黑莓栽培品种的光合作用能力

背景：最近，盐胁迫已成为对作物生产产生负面影响的最重要非生物胁迫之一。光合作用是植物的一个重要生化过程，对包括盐胁迫在内的环境限制非常敏感。目的：本研究旨在评估盐胁迫对黑莓（Rubus fructicosus L.）叶片光系统 II 的影响。方法：将两个黑莓栽培品种（Thornfree 和 Boysen）置于盐胁迫（20、40 和 60 mM NaCl）下进行 28 天的离体培养。通过检测叶绿素 a 荧光（JIP 试验）和光合色素来评估光合作用活性。结果：盐胁迫对博伊森（Boysen）栽培品种叶片的 PSII 最大量子产率（Fv/Fm）、性能指标（PIABS 和 PItotal）和光合驱动力（DFABS）有很大影响。每 RC 吸收通量（ABS/RC）、每 PSII RC 捕获通量（TR0/RC）、每 RC 耗散通量（DI0/RC）的增加值、叶绿素和类胡萝卜素含量的降低值、光收集复合物与 PSII 核心蛋白之间相互作用的破坏以及氧发生复合物的破坏都证实了光合效率的降低。结论：所提供的信息表明，与博伊森相比，Thornfree 栽培品种的光合装置对盐胁迫的耐受性更强。

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来源期刊

Journal of Berry Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

3.50

自引率

11.80%

发文量

期刊介绍： The main objective of the Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems. The objective will be achieved by focusing on four main areas of research and development: From genetics to variety evaluation Nursery production systems and plant quality control Plant physiology, biochemistry and molecular biology, as well as cultural management Health for the consumer: components and factors affecting berries'' nutritional value Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.