atcog8-2, A New Mutant Allele of the Conserved Oligomeric Golgi Complex 8, Reveals the Need for the COG Complex for Gametophyte Development in Arabidopsis
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引用次数: 0
Abstract
Angiosperms proliferate through double fertilization mediated by male (pollen) and female (embryo sac) gametophytes. To determine the genes essential for pollen development in Arabidopsis thaliana, we first generated a mutant population using an activation tagging vector with herbicide-resistance gene and screened mature pollen phenotypes. Then, a T-DNA insertional heterozygous line was isolated, initially named AP22-48, which produced high levels of abnormal pollen grains. Reciprocal crosses revealed that the genetic transmission of the mutant allele was completely blocked through the male and was highly limited through the female. Determination of T-DNA flanking sequences and genetic complementation of AP22-48 identified AtCOG8, a subunit of the Conserved Oligomeric Golgi (COG) complex, which is a tethering factor essential for the Golgi architecture and retrograde vesicle trafficking in eukaryotes. We renamed the mutant atcog8-2, with reference to a previous cog8 mutant (atcog8-1). While atcog8-1 induced male-specific defects during pollen tube growth, atcog8-2 mutant failed to produce normal gametophytes in both sexes. Detailed morphological analysis demonstrated aberrant development of the pollen and embryo sac in atcog8-2 mutants. This study, thus, strongly suggests that the COG complex functions are broad and indispensable for accurate gametophyte development, which is a prerequisite for sexual reproduction in Arabidopsis.
期刊介绍：
Journal of Plant Biology, an official publication of the Botanical Society of Korea, is an international journal devoted to basic researches in biochemistry, cellular biology, development, ecology, genetics, molecular biology, physiology, and systematics of plants.
The Journal publishes the following categories of paper:
Original articles -- For publication in Journal of Plant Biology the manuscript must provide a significant new contribution to our understanding of plants. All areas of plant biology are welcome. No limit on the length, but a concise presentation is encouraged.
Reviews -- Invited by the EiC.
Brief Communications -- Concise but independent report representing significant contribution to plant science.
The Botanical Society of Korea was founded on November 30, 1957 to promote studies, disseminate and exchange information on the field of plant biology. The first issue of The Korean Journal of Botany, the official publication of the society, was published on April 1, 1958. It was published twice a year, but quarterly from 5th volume in 1962. In 1994, it was renamed to Journal of Plant Biology and published in English since 1996. The journal entered its 50th year of publication in 2007.
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