Immunocytochemistry on frozen-embedded cell block for the diagnosis of hematolymphoid cytology specimen: a straightforward alternative to the conventional cell block

Pub Date : 2023-12-15 DOI:10.1007/s12308-023-00569-0

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Abstract

Agarose-based cell block (CB) technique can be modified to be combined with the frozen section technique for the preparation of a high-quality frozen-embedded CB (F-CB) from an effusion or fine-needle aspiration (FNA) cytology sample. This combined technique can be effectively used for the immunocharacterization of the hematolymphoid cells on F-CB. To demonstrate the applicability of performing diagnostic ICC on F-CB, we have analyzed the immunophenotype of the hematolymphoid cells in a series of eight cases of effusions and eight cases of FNA cytology specimens by using CB-ICC on sections cut from frozen-embedded CBs. The SurePath^TM residue or cytologic material scraped off from the FNA cytology smear that was diagnostic for or suspicious of hematolymphoid malignancy was pelleted and pre-embedded in agarose. Half of the agarose-embedded pellet was frozen-embedded in OCT compound for the preparation of F-CB, while the other half was processed for the preparation of paraffin-embedded CB. Sections cut from the F-CB and P-CB were used for CB-ICC. Panels of ICC on the F-CBs could enable the immunocytochemical differential diagnosis of large cell hematologic malignancies that encompass anaplastic large cell lymphoma and other forms of large-cell hematolymphoid malignancies such as large B-cell lymphomas, anaplastic plasma cell myeloma, myeloid sarcoma, and T-lymphoblastic lymphoma. It also appeared that the small B-cell lymphomas in the effusions or FNAs could be differentially diagnosed with the aid of CB-ICC on the F-CB. A modified agarose-based CB technique can be combined with the frozen-embedded CB method for the preparation of F-CB that can be directly used for the immunocytochemical differential diagnosis of hematolymphoid cytology samples.

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在冷冻包埋细胞块上进行免疫细胞化学分析以诊断血淋巴细胞学标本：传统细胞块的直接替代方法

摘要基于琼脂糖的细胞块（CB）技术可与冷冻切片技术相结合，从渗出液或细针穿刺（FNA）细胞学样本中制备高质量的冷冻包埋 CB（F-CB）。这种组合技术可有效用于对 F-CB 上的血淋巴细胞进行免疫定性。为了证明在 F-CB 上进行诊断性 ICC 的适用性，我们使用 CB-ICC 对冷冻埋藏的 CB 切片进行了一系列分析，对 8 例渗出液和 8 例 FNA 细胞学样本中的血淋巴细胞进行了免疫表型分析。将 SurePathTM 残留物或从 FNA 细胞学涂片上刮下的可诊断或怀疑为血淋巴细胞恶性肿瘤的细胞学材料颗粒化，并预先包埋在琼脂糖中。琼脂糖包埋颗粒的一半冷冻包埋在 OCT 化合物中，用于制备 F-CB，另一半用于制备石蜡包埋 CB。从 F-CB 和 P-CB 上切下的切片用于 CB-ICC 分析。F-CB上的ICC切片可用于大细胞血液恶性肿瘤的免疫细胞化学鉴别诊断，其中包括无性大细胞淋巴瘤和其他形式的大细胞血淋巴恶性肿瘤，如大B细胞淋巴瘤、无性浆细胞骨髓瘤、骨髓肉瘤和T淋巴细胞淋巴瘤。此外，借助 F-CB 上的 CB-ICC 技术，似乎还可以对渗出液或 FNA 中的小 B 细胞淋巴瘤进行鉴别诊断。改良的琼脂糖基 CB 技术可与冷冻包埋 CB 方法相结合制备 F-CB，直接用于血淋巴细胞样本的免疫细胞化学鉴别诊断。

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