Full-length genome characterization of a novel mitovirus isolated from the root rot fungus Armillaria mellea

IF 1.9 4区 医学 Q3 GENETICS & HEREDITY Virus Genes Pub Date : 2023-12-16 DOI:10.1007/s11262-023-02041-8
Sıla Erkmen, Ergin Sahin, Ilgaz Akata
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Abstract

Members of the genus Armillaria belong to the group of pathogenic and facultative saprotrophic fungi that are generally known as one of the causative agents of white root rot in infected plants including deciduous and evergreen trees and shrubs. Although several single-stranded RNA mycoviruses were previously described in different Armillaria species, there is no report on mitoviruses (one of the simplest RNA viruses of fungal hosts) known to infect Armillaria taxa. In this study, a new mitovirus denominated “Armillaria mellea mitovirus 1” (AmMV1) was identified in the sporophore samples of Armillaria mellea, commonly known as honey mushroom. AmMV1 has a genome length of 4440 nucleotides and a G + C content of 48%. It encompasses a single open reading frame (ORF) that encodes an RNA-dependent RNA polymerase (RdRp). Comparison through BLASTp analysis revealed that the RdRp domain of AmMV1 shares a sequence identity ranging from 33.43% to 43.27% with RdRp domains of Duamitovirus genus members, having the highest similarity (43.27%) to Rhizoctonia solani mitovirus 94. According to phylogenetic analysis, AmMV1 is classified as a member of the genus Duamitovirus belonging to the Mitoviridae family. This marks the initial instance of a mitovirus identified in Armillaria spp..

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从根腐菌 Armillaria mellea 中分离出的新型丝状病毒的全长基因组特征描述
节肢动物真菌属(Armillaria)的成员属于致病真菌和兼性嗜渍真菌,通常是受感染植物(包括落叶和常绿乔灌木)白根腐病的病原体之一。虽然以前曾描述过几种单链 RNA 真菌病毒,但目前还没有关于丝核病毒(真菌宿主中最简单的 RNA 病毒之一)感染石珊瑚类群的报道。本研究在俗称为蜜菇的 Armillaria mellea 孢子体样本中发现了一种新的丝状病毒,命名为 "Armillaria mellea mitovirus 1"(AmMV1)。AmMV1 的基因组长度为 4440 个核苷酸,G+C 含量为 48%。它包含一个开放阅读框(ORF),编码一种 RNA 依赖性 RNA 聚合酶(RdRp)。通过 BLASTp 分析比较发现,AmMV1 的 RdRp 结构域与 Duamitovirus 属成员的 RdRp 结构域具有 33.43% 至 43.27% 的序列同一性,其中与 Rhizoctonia solani mitovirus 94 的相似性最高(43.27%)。根据系统进化分析,AmMV1 被归类为属于丝状病毒科 Duamitovirus 属的成员。这标志着首次在刺芹属植物中发现了丝状病毒。
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来源期刊
Virus Genes
Virus Genes 医学-病毒学
CiteScore
3.30
自引率
0.00%
发文量
76
审稿时长
3 months
期刊介绍: Viruses are convenient models for the elucidation of life processes. The study of viruses is again on the cutting edge of biological sciences: systems biology, genomics, proteomics, metagenomics, using the newest most powerful tools. Huge amounts of new details on virus interactions with the cell, other pathogens and the hosts – animal (including human), insect, fungal, plant, bacterial, and archaeal - and their role in infection and disease are forthcoming in perplexing details requiring analysis and comments. Virus Genes is dedicated to the publication of studies on the structure and function of viruses and their genes, the molecular and systems interactions with the host and all applications derived thereof, providing a forum for the analysis of data and discussion of its implications, and the development of new hypotheses.
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