Ultra-high-throughput mass spectrometry in drug discovery: fundamentals and recent advances.

IF 6 2区 医学 Q1 PHARMACOLOGY & PHARMACY Expert Opinion on Drug Discovery Pub Date : 2024-03-01 Epub Date: 2023-12-19 DOI:10.1080/17460441.2023.2293153
Jon D Williams, Fan Pu, James W Sawicki, Nathaniel L Elsen
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Abstract

Introduction: Ultra-high-throughput mass spectrometry, uHT-MS, is a technology that utilizes ionization and sample delivery technologies optimized to enable sampling from well plates at > 1 sample per second. These technologies do not need a chromatographic separation step and can be utilized in a wide variety of assays to detect a broad range of analytes including small molecules, lipids, and proteins.

Areas covered: This manuscript provides a brief historical review of high-throughput mass spectrometry and the recently developed technologies that have enabled uHT-MS. The report also provides examples and references on how uHT-MS has been used in biochemical and chemical assays, nuisance compound profiling, protein analysis and high throughput experimentation for chemical synthesis.

Expert opinion: The fast analysis time provided by uHT-MS is transforming how biochemical and chemical assays are performed in drug discovery. The potential to associate phenotypic responses produced by 1000's of compound treatments with changes in endogenous metabolite and lipid signals is becoming feasible. With the augmentation of simple, fast, high-throughput sample preparation, the scope of uHT-MS usage will increase. However, it likely will not supplant LC-MS for analyses that require low detection limits from complex matrices or characterization of complex biotherapeutics such as antibody-drug conjugates.

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药物发现中的超高通量质谱法:基本原理和最新进展。
简介超高通量质谱(uHT-MS)是一种利用电离和样品传输技术的技术,该技术经过优化,能够以大于每秒 1 个样品的速度从孔板取样。这些技术不需要色谱分离步骤,可用于多种检测方法,检测包括小分子、脂类和蛋白质在内的多种分析物:本手稿简要回顾了高通量质谱法的历史以及最近开发的 uHT-MS 技术。报告还提供了有关 uHT-MS 如何用于生化和化学分析、有害化合物分析、蛋白质分析和化学合成高通量实验的实例和参考文献:uHT-MS提供的快速分析时间正在改变药物发现中生化和化学分析的方式。将 1000 种化合物处理所产生的表型反应与内源性代谢物和脂质信号的变化联系起来的可能性正在变得可行。随着简单、快速、高通量样品制备技术的发展,uHT-MS 的应用范围将会扩大。不过,对于需要从复杂基质中获得低检测限的分析或复杂生物治疗药物(如抗体-药物共轭物)的表征,uHT-MS 可能无法取代 LC-MS。
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来源期刊
CiteScore
10.20
自引率
1.60%
发文量
78
审稿时长
6-12 weeks
期刊介绍: Expert Opinion on Drug Discovery (ISSN 1746-0441 [print], 1746-045X [electronic]) is a MEDLINE-indexed, peer-reviewed, international journal publishing review articles on novel technologies involved in the drug discovery process, leading to new leads and reduced attrition rates. Each article is structured to incorporate the author’s own expert opinion on the scope for future development. The Editors welcome: Reviews covering chemoinformatics; bioinformatics; assay development; novel screening technologies; in vitro/in vivo models; structure-based drug design; systems biology Drug Case Histories examining the steps involved in the preclinical and clinical development of a particular drug The audience consists of scientists and managers in the healthcare and pharmaceutical industry, academic pharmaceutical scientists and other closely related professionals looking to enhance the success of their drug candidates through optimisation at the preclinical level.
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