Evaluation of Ki67, Bax, Bcl-2 and KIT in canine cutaneous mast cell tumours and their relationship with histopathology and prognosis.

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES Acta veterinaria Hungarica Pub Date : 2023-12-18 Print Date: 2024-01-09 DOI:10.1556/004.2023.00867
Işıl Aytemiz Danyer, Aydın Gürel
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引用次数: 0

Abstract

Canine cutaneous mast cell tumours (CCMCTs) are common in dogs and exhibit many unpredictable behaviors. This study aimed to encourage pathology laboratories in developing countries to routinely assess prognosis by applying commonly used histopathological grading systems and immunohistochemistry (IHC) markers. We performed histological grading according to both the Patnaik and Kiupel systems, determined the mitotic count (MC) and carried out IHC for the detection of Ki67, Bax, Bcl-2 and KIT in 54 CCMCT cases. MC was associated with both grading systems in terms of survival following diagnosis and prognostic factors differed among cases categorized by the cut-off value of 5. KIT patterns were associated with grading systems and MC. The cohort with pattern II had a lower survival rate than those with patterns I and III. Ki67 was associated with survival when evaluated over the cut-off value of 0.018. Bax expression was associated with both grading systems. Median survival time was longer in patients with lower Bax expression level. Immunohistochemical detection of KIT, Ki67 and Bax improves histopathology in predicting the prognosis. If IHC is unavailable, reports regarding MC and values from both grading systems are the most effective, convenient and cost-effective way to provide the most reliable prognostic data and guidance for the clinicians.

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犬皮肤肥大细胞瘤中 Ki67、Bax、Bcl-2 和 KIT 的评估及其与组织病理学和预后的关系。
犬皮肤肥大细胞瘤(CCMCT)在犬中很常见,并表现出许多不可预测的行为。本研究旨在鼓励发展中国家的病理实验室通过应用常用的组织病理学分级系统和免疫组织化学(IHC)标记来常规评估预后。我们根据 Patnaik 和 Kiupel 系统对 54 例 CCMCT 进行了组织学分级,确定了有丝分裂计数(MC),并对 Ki67、Bax、Bcl-2 和 KIT 进行了 IHC 检测。就确诊后的生存率而言,MC 与两种分级系统均有关联,而以 5 为临界值分类的病例之间的预后因素存在差异。KIT 模式与分级系统和 MC 相关。与模式 I 和 III 患者相比,模式 II 患者的生存率较低。当截断值为0.018时,Ki67与存活率相关。Bax的表达与两种分级系统都有关系。Bax表达水平较低的患者中位生存时间较长。免疫组化检测 KIT、Ki67 和 Bax 可改善组织病理学对预后的预测。如果没有 IHC,两种分级系统的 MC 和数值报告是为临床医生提供最可靠的预后数据和指导的最有效、最方便和最具成本效益的方法。
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来源期刊
Acta veterinaria Hungarica
Acta veterinaria Hungarica 农林科学-兽医学
CiteScore
1.80
自引率
0.00%
发文量
39
审稿时长
>36 weeks
期刊介绍: Acta Veterinaria Hungarica publishes original research papers presenting new scientific results of international interest, and to a limited extent also review articles and clinical case reports, on veterinary physiology (physiological chemistry and metabolism), veterinary microbiology (bacteriology, virology, immunology, molecular biology), on the infectious diseases of domestic animals, on veterinary parasitology, pathology, clinical veterinary science and reproduction.
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