Genotoxic and Cytotoxic Effects of Cone Beam Computed Tomography and Multidetector Computed Tomography on Exfoliated Buccal Epithelial Cells.

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL Iranian Journal of Medical Sciences Pub Date : 2023-11-01 DOI:10.30476/IJMS.2023.96522.2807
Zahra Jahanshahiafshar, Hakimeh Ghorbani, Maryam Seyedmajidi, Mehrdad Nabahati, Kourosh Ebrahimnejad Gorji, Seyedali Seyedmajidi, Ehsan Moudi
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引用次数: 0

Abstract

Background: Cone beam computed tomography (CBCT) and multidetector computed tomography (MDCT) are frequently used in dental and maxillofacial problems. This study aimed to assess the genotoxicity and cytotoxicity effects of CBCT and MDCT radiographies on exfoliated buccal epithelial cells during dental examinations.

Methods: This prospective experimental study was conducted at Babol University of Medical Sciences (Babol, Iran) from March 2021 to April 2021. Buccal mucosa smears were collected bilaterally pre-exposure and 12 days after CBCT or MDCT examinations. To compare the frequency of micronuclei and other cytotoxic cellular changes such as pyknosis, karyolysis, and karyorrhexis, the paired sample t test and Wilcoxon test were used. In addition, independent sample t test, Mann-Whitney, and Chi square tests were used to investigate the differences between the imaging methods and between men and women. All statistical analyses were performed using the SPSS software, and P≤0.05 was considered statistically significant.

Results: The current study included 60 adult patients (30 patients in each group), ranging in age from 21 to 50 years. The micronuclei and the other cytotoxic cellular changes increased significantly after CBCT and MDCT radiographic examinations on the 12th day compared to the pre-exposure results (P<0.001). MDCT had statistically higher cytotoxic and genotoxic effects than CBCT (9.4%, 23.1%, and 40% higher values in micronucleus frequency, the mean frequency of micronuclei, and other cytotoxic changes, respectively). There were no significant differences between men and women in the two examination methods (P=0.46 and P=0.49, respectively).

Conclusion: Dental examinations with CBCT and MDCT can increase cytotoxicity and chromosomal damage in both men and women. Due to its lower radiation toxicities, CBCT can be recommended as an alternative to MDCT for dental examinations.

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锥形束计算机断层扫描和多载体计算机断层扫描对脱落颊上皮细胞的基因毒性和细胞毒性效应
背景:锥形束计算机断层扫描(CBCT)和多载体计算机断层扫描(MDCT)经常用于牙科和颌面部问题。本研究旨在评估 CBCT 和 MDCT 放射摄影对牙科检查过程中脱落的口腔上皮细胞的遗传毒性和细胞毒性影响:这项前瞻性实验研究于 2021 年 3 月至 2021 年 4 月在巴博勒医科大学(伊朗巴博勒)进行。在接触前和 CBCT 或 MDCT 检查后 12 天收集双侧颊粘膜涂片。为了比较微核和其他细胞毒性细胞变化(如细胞凋亡、核溶解和核分裂)的频率,采用了配对样本 t 检验和 Wilcoxon 检验。此外,还使用独立样本 t 检验、曼-惠特尼检验和卡方检验来研究不同成像方法之间以及男女之间的差异。所有统计分析均使用 SPSS 软件进行,P≤0.05 为差异有统计学意义:本次研究包括 60 名成年患者(每组 30 人),年龄从 21 岁到 50 岁不等。与暴露前的结果相比,第 12 天进行 CBCT 和 MDCT 放射检查后,微核和其他细胞毒性变化明显增加(PC 结论:CBCT 和 MDCT 放射检查的结果与暴露前的结果相比,微核和其他细胞毒性变化明显增加:使用 CBCT 和 MDCT 进行牙科检查会增加男性和女性的细胞毒性和染色体损伤。由于 CBCT 的辐射毒性较低,建议在牙科检查中使用 CBCT 替代 MDCT。
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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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