Molecular Serotyping and Antibiotic Resistance Profile of Group B Streptococcus Strains Isolated from Iranian Pregnant Women with Urinary Tract Infection.

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL Iranian Journal of Medical Sciences Pub Date : 2023-11-01 DOI:10.30476/ijms.2023.96346.2787
Natalie Gharabeigi, Akram Sadat Tabatabaee Bafroee, Kumarss Amini
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Abstract

Background: Group B Streptococcus (GBS) can cause serious infections in neonates and pregnant women. GBS may cause urinary tract infections (UTIs). However, molecular epidemiology of such infections is rarely reported. The present study aimed to determine drug resistance patterns and molecular serotyping of GBS isolates in a population of pregnant Iranian women with UTIs.

Methods: A cross-sectional study was conducted during the first half of 2021 in the Department of Biology, East Tehran Branch, Islamic Azad University (Tehran, Iran). Sixty GBS strains isolated from the urine and placenta samples of pregnant women with UTIs were evaluated. The women were aged 19-46 years old at 35 to 37 weeks of gestation. The molecular serotype of GBS isolates was determined using a multiplex polymerase chain reaction, and the disc diffusion method was used to determine the antibiotic susceptibility pattern of isolates for different antibiotics. The association of the GBS serotype with the phenotype of antibiotic resistance was statistically analyzed using SPSS software (version 22.0) with a Chi square test and Cramer's V test. P<0.05 was considered statistically significant.

Results: GBS capsular serotype II was most prevalent (66.7%) followed by serotypes Ib (21.7%), Ia (3.3%), and III (1.7%). The prevalence of non-typeable isolates was significantly low (6.6%). Of the 60 GBS isolates, 18.3% were resistant to penicillin, 81.6% to ampicillin, 23.3% to clindamycin, and 30% to vancomycin; indicating the need for treatment alternatives.

Conclusion: Region-specific information on antibiotic resistance and molecular characteristics of GBS is essential for epidemiological investigations, effective treatment, and vaccine development.

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从患有尿路感染的伊朗孕妇中分离出的 B 组链球菌菌株的分子血清型和抗生素耐药性概况。
背景:B 组链球菌 (GBS) 可导致新生儿和孕妇发生严重感染。GBS 可能会引起尿路感染(UTI)。然而,此类感染的分子流行病学却鲜有报道。本研究旨在确定伊朗孕妇尿路感染人群中 GBS 分离物的耐药模式和分子血清型:2021 年上半年,伊朗阿扎德伊斯兰大学东德黑兰分校生物系(伊朗德黑兰)开展了一项横断面研究。对从患有尿毒症的孕妇的尿液和胎盘样本中分离出的 60 株 GBS 进行了评估。这些孕妇的年龄在 19-46 岁之间,孕周在 35-37 周之间。使用多重聚合酶链反应确定了分离出的 GBS 分子血清型,并使用盘扩散法确定了分离出的菌株对不同抗生素的敏感性模式。使用 SPSS 软件(22.0 版)对 GBS 血清型与抗生素耐药表型的相关性进行了统计分析,并进行了 Chi Square 检验和 Cramer's V 检验。结果GBS 胶囊血清型 II 最常见(66.7%),其次是血清型 Ib(21.7%)、Ia(3.3%)和 III(1.7%)。不可分型分离物的流行率明显较低(6.6%)。在 60 个 GBS 分离物中,18.3% 对青霉素耐药,81.6% 对氨苄西林耐药,23.3% 对克林霉素耐药,30% 对万古霉素耐药;这表明需要替代治疗方法:结论:有关 GBS 抗生素耐药性和分子特征的特定地区信息对于流行病学调查、有效治疗和疫苗开发至关重要。
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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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