Association of MGLL Intronic C>T Single Nucleotide Polymorphism (rs782440) with Borderline Personality Disorder: A Case-Control Study.

IF 1.7 4区 生物学 Q4 CELL BIOLOGY Cell Journal Pub Date : 2023-11-28 DOI:10.22074/cellj.2023.2004323.1321
Nazanin Hatami Bavarsad, Leila Jahangard, Masood Saidijam, Seyed Asaad Karimi, Ali Reza Soltanian, Elahe Shahriari, Saeid Afshar, Abdolrahman Sarihi
{"title":"Association of <i>MGLL</i> Intronic C>T Single Nucleotide Polymorphism (rs782440) with Borderline Personality Disorder: A Case-Control Study.","authors":"Nazanin Hatami Bavarsad, Leila Jahangard, Masood Saidijam, Seyed Asaad Karimi, Ali Reza Soltanian, Elahe Shahriari, Saeid Afshar, Abdolrahman Sarihi","doi":"10.22074/cellj.2023.2004323.1321","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>From the perspective of etiology, borderline personality disorder (BPD) is a multifactorial and complex disorder, hence our understanding about the molecular basis and signaling of this disorder is extremely limited. The purpose of this study was evaluating the relationship between BPD and the Monoacylglycerol lipase <i>(MGLL)</i> polymorphism rs782440 in the population of Hamadan, Iran.</p><p><strong>Materials and methods: </strong>In this case-control study, 106 participants including 53 patients with BPD and 53 healthy control subjects were selected by psychiatrists in the Department of Psychiatry at Farshchian Sina Hospital in Hamadan. The BPD patients were selected based on the Diagnostic and Statistical Manual of Mental Disorders <i>(DSM-5)</i> form for diagnosing BPD patients. For genotyping, polymerase chain reaction (PCR) was used to amplify the desired region including the <i>(MGLL)</i> intronic C>T single nucleotide polymorphism (SNP) (rs782440) and afterward the amplicon was sequenced using the Sanger sequencing method. To determine the genotype of these patients, their sequences were aligned with the reference sequence of MGLL through the CLC genomic workbench software.</p><p><strong>Results: </strong>The results indicated that the frequency of TT in comparison to the CC genotype was significantly different (P=0.003) and the risk of BPD in change from the TT genotype to CC genotype was increased by 6.679%. Regarding the frequency of allele in this group, no significant difference was observed.</p><p><strong>Conclusion: </strong>This paper, has studied and reports for the first time, the association between <i>MGLL</i> SNP (rs782440) with BPD. The findings of the current research revealed that the TT genotype increases the risk of BPD compared to the CC genotype. Considering the lack of a suitable diagnostic biomarker for BPD, using this potential biomarker in the near future can be promising.</p>","PeriodicalId":49224,"journal":{"name":"Cell Journal","volume":"25 11","pages":"783-789"},"PeriodicalIF":1.7000,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10711293/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Journal","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.22074/cellj.2023.2004323.1321","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: From the perspective of etiology, borderline personality disorder (BPD) is a multifactorial and complex disorder, hence our understanding about the molecular basis and signaling of this disorder is extremely limited. The purpose of this study was evaluating the relationship between BPD and the Monoacylglycerol lipase (MGLL) polymorphism rs782440 in the population of Hamadan, Iran.

Materials and methods: In this case-control study, 106 participants including 53 patients with BPD and 53 healthy control subjects were selected by psychiatrists in the Department of Psychiatry at Farshchian Sina Hospital in Hamadan. The BPD patients were selected based on the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) form for diagnosing BPD patients. For genotyping, polymerase chain reaction (PCR) was used to amplify the desired region including the (MGLL) intronic C>T single nucleotide polymorphism (SNP) (rs782440) and afterward the amplicon was sequenced using the Sanger sequencing method. To determine the genotype of these patients, their sequences were aligned with the reference sequence of MGLL through the CLC genomic workbench software.

Results: The results indicated that the frequency of TT in comparison to the CC genotype was significantly different (P=0.003) and the risk of BPD in change from the TT genotype to CC genotype was increased by 6.679%. Regarding the frequency of allele in this group, no significant difference was observed.

Conclusion: This paper, has studied and reports for the first time, the association between MGLL SNP (rs782440) with BPD. The findings of the current research revealed that the TT genotype increases the risk of BPD compared to the CC genotype. Considering the lack of a suitable diagnostic biomarker for BPD, using this potential biomarker in the near future can be promising.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
MGLL Intronic C>T 单核苷酸多态性(rs782440)与边缘型人格障碍的关系:病例对照研究
研究目的从病因学的角度来看,边缘型人格障碍(BPD)是一种多因素的复杂障碍,因此我们对这种障碍的分子基础和信号转导的了解非常有限。本研究的目的是评估伊朗哈马丹地区人群中 BPD 与单酰基甘油脂肪酶 (MGLL) 多态性 rs782440 之间的关系:在这项病例对照研究中,哈马丹 Farshchian Sina 医院精神科的精神科医生挑选了 106 名参与者,其中包括 53 名 BPD 患者和 53 名健康对照者。BPD患者是根据《精神疾病诊断与统计手册》(DSM-5)中诊断BPD患者的表格选出的。在进行基因分型时,使用聚合酶链式反应 (PCR) 扩增所需区域,包括 (MGLL) 内含子 C>T 单核苷酸多态性 (SNP)(rs782440),然后使用桑格测序法对扩增子进行测序。为了确定这些患者的基因型,通过 CLC genomic workbench 软件将他们的序列与 MGLL 的参考序列进行了比对:结果表明,TT 基因型与 CC 基因型的频率有显著差异(P=0.003),从 TT 基因型转变为 CC 基因型时,罹患 BPD 的风险增加了 6.679%。关于该组等位基因的频率,没有观察到明显差异:本文首次研究并报告了 MGLL SNP(rs782440)与 BPD 之间的关联。研究结果表明,与 CC 基因型相比,TT 基因型会增加罹患 BPD 的风险。考虑到目前还没有合适的 BPD 诊断生物标志物,在不久的将来使用这一潜在的生物标志物将大有可为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
相关文献
Book List
IF 0.9 4区 环境科学与生态学Natural Areas JournalPub Date : 2016-01-01 DOI: 10.3375/043.036.0216
Book List
IF 0.9 4区 环境科学与生态学Natural Areas JournalPub Date : 2017-01-01 DOI: 10.3375/043.037.0316
BooK LisT
BOOK LIST
IF 0.1 4区 哲学HEYTHROP JOURNALPub Date : 1963-07-01 DOI: 10.1111/j.1468-2265.1963.tb00322.x
来源期刊
Cell Journal
Cell Journal CELL BIOLOGY-
CiteScore
3.40
自引率
5.00%
发文量
0
审稿时长
12 months
期刊介绍: The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.
期刊最新文献
Oxaliplatin-Loaded Chitosan Nanoparticles Decorated with Cetuximab Single-Chain Variable Fragment for Human Colorectal Cancer Treatment. The Geographical Distribution of Global Biobanks. The Role of Quercetin and Exercise in Modulating Apoptosis and Cardiomyopathy via PI3K/AKT/FOXO3 Pathways in Diabetic Obese Rats. Cell-Based Therapy for Cerebral Palsy: A Puzzle in Progress. Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1