The Effect of Mesenchymal Stem Cells Derived-Conditioned Media in Combination with Oral Anti-Androgenic Drugs on Male Pattern Baldness: An Animal Study.
Majid Kamali-Dolat Abadi, Gholamhossein Yousefi, Farzaneh Dehghani, Ali Akbar Alizadeh, Abolfazl Jangholi, Mohammad Amin Moadab, Maryam Naseh, Shima Parsa, Golara Nasiri, Negar Azarpira, Mehdi Dianatpour
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引用次数: 0
Abstract
Objective: Androgenetic alopecia (AGA) is a prevalent form of hair loss, mainly caused by follicular sensitivity to androgens. Despite developing different anti-androgen treatment options, the success rate of these treatments has been limited. Using animal models, this study evaluated the therapeutic effects of umbilical cord (UC) stem cell conditioned media (CM) combined with oral anti-androgens for hair regeneration.
Materials and methods: In this experimental study, Poloxamer 407 (P407) was used as a drug carrier for subcutaneous testosterone injection. AGA models were treated with oral finasteride, oral flutamide, and CM injections. Samples were thoroughly evaluated and compared using histological, stereological, and molecular analyses.
Results: Injecting CM-loaded hydrogel alone or combined with oral intake of anti-androgens improved hair regeneration. These treatments could promote hair growth by inducing hair follicles in the anagen stage and shortening the telogen and catagen phases. Furthermore, the combination treatment led to an upregulation of hair induction gene expression with a downregulation of inflammation genes.
Conclusion: Through a reduction in inflammation, injection of CM-loaded hydrogel alone or combined with oral intake of anti-androgens induces the hair cell cycle with regeneration in damaged follicles. Hence, this could be a promising therapeutic method for AGA patients.
目的：雄激素性脱发（AGA）是一种常见的脱发形式，主要由毛囊对雄激素敏感引起。尽管开发了不同的抗雄激素治疗方案，但这些治疗方法的成功率有限。本研究利用动物模型，评估了脐带（UC）干细胞条件培养基（CM）结合口服抗雄激素对毛发再生的治疗效果：在这项实验研究中，Poloxamer 407（P407）被用作皮下注射睾酮的药物载体。AGA 模型接受了口服非那雄胺、口服氟他胺和中药注射治疗。通过组织学、立体学和分子分析对样本进行了全面评估和比较：结果：单独或结合口服抗雄激素注射 CM 水凝胶都能改善毛发再生。这些疗法可通过诱导处于生长期的毛囊，缩短毛囊的休止期和衰退期来促进毛发生长。此外，联合疗法还能上调毛发诱导基因的表达，同时下调炎症基因的表达：结论：通过减少炎症，单独注射或与口服抗雄激素结合注射含有 CM 的水凝胶可诱导毛细胞周期，促进受损毛囊的再生。因此，这可能是治疗 AGA 患者的一种有前途的方法。
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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