MESENCHYMAL CELLS IN ROTATOR CUFF REPAIR - TECHNIQUE DESCRIPTION AND CASE REPORTS.

IF 0.5 4区医学 Q4 ORTHOPEDICS Acta Ortopedica Brasileira Pub Date : 2023-12-18 eCollection Date: 2023-01-01 DOI:10.1590/1413-785220233105e268392

Eduardo Angeli Malavolta, Vinicius Lamboglia Miceli, Jorge Henrique Assunção, Fernando Brandao Andrade-Silva, Mauro Emilio Conforto Gracitelli, Nelson Hidekazu Tatsui, Luiz César Espirandelli, Arnaldo Amado Ferreira

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Abstract

Objective: To describe a protocol of obtention of mesenchymal stem cells and to report their use as a biological adjuvant in three patients undergoing arthroscopic rotator cuff repair.

Methods: Case series of patients who underwent arthroscopic repair of isolated full-thickness supraspinatus tear using mesenchymal stem cells obtained from the bone marrow as a biological adjuvant. All patients were operated on at the same institution, by a surgeon with 13 years of experience. The cells were applied at the end of the procedure, at the tendon-bone interface, at an approximate concentration of 2,000,000 mesenchymal cells/mm³ and a total volume of 5 ml.

Results: All patients improved with the procedure, with one excellent and two good results. All cases overcame the minimally important clinical difference. All cases reached tendon healing, without partial or complete re-tears. We observed no complications.

Conclusion: Arthroscopic rotator cuff repair with added mesenchymal cells obtained from bone marrow and submitted to a cell expansion process led to good functional results and healing in all cases in the sample, with no complications. Level of Evidence IV, Case Series.

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间充质细胞在肩袖修复中的应用--技术描述和病例报告。

目的描述间充质干细胞的获取方案，并报告间充质干细胞在三名接受关节镜肩袖修复术的患者中作为生物佐剂的应用情况：方法：使用从骨髓中获得的间充质干细胞作为生物辅助剂，对接受关节镜下孤立性全厚度冈上肌撕裂修复术的患者进行病例系列研究。所有患者均在同一家医疗机构接受手术，由一位拥有13年经验的外科医生主刀。细胞在手术结束后应用于肌腱与骨骼的交界处，浓度约为 2,000,000 间充质干细胞/立方毫米，总容量为 5 毫升：结果：所有患者的疗效都有所改善，其中一名患者疗效极佳，两名患者疗效良好。所有病例都克服了最小临床差异。所有病例都实现了肌腱愈合，没有部分或完全再次撕裂。我们没有观察到并发症：结论：添加了骨髓间充质细胞并经过细胞扩增过程的肩袖关节镜修复术在所有样本病例中均取得了良好的功能效果和愈合，且无并发症。证据等级IV，病例系列。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Ortopedica Brasileira Orthopedics-

CiteScore

0.90

自引率

14.30%

发文量

审稿时长

25 weeks

期刊介绍： A Revista Acta Ortopédica Brasileira, órgão oficial do Departamento de Ortopedia e Traumatologia da Faculdade de Medicina da Universidade de São Paulo (DOT/FMUSP), é publicada bimestralmente em seis edições ao ano (jan/fev, mar/abr, maio/jun, jul/ago, set/out e nov/dez) com versão em inglês disponível nos principais indexadores nacionais e internacionais e instituições de ensino do Brasil. Sendo hoje reconhecidamente uma importante contribuição para os especialistas da área com sua seriedade e árduo trabalho para as indexações já conquistadas.