Enhanced growth and metabolite production from a novel strain of Porphyridium sp.

IF 4.2 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioengineered Pub Date : 2024-12-01 Epub Date: 2023-12-22 DOI:10.1080/21655979.2023.2294160
Latifa Tounsi, Hajer Ben Hlima, Hana Derbel, David Duchez, Christine Gardarin, Pascal Dubessay, Marwa Drira, Imen Fendri, Philippe Michaud, Slim Abdelkafi
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Abstract

Microalgae are capable of generating numerous metabolites that possess notable biological activities and hold substantial promise for various industrial applications. Nevertheless, the taxonomic diversity of these photosynthetic microorganisms has not received thorough investigation. Using the 18S rRNA encoding gene, a recently discovered strain originating from the Tunisian coast (the governorate of Mahdia) was identified as a member of the Porphyridium genus. The growth response as well as the metabolite accumulation of Porphyridium sp. to different culture media (Pm, F/2, and Hemerick) was investigated over a period of 52 days. The highest biomass production was recorded with Pm medium (2 × 107 cell/mL). The apparent growth rates (µ) and the doubling time (Dt) were about 0.081 day-1 and 12.34 days, respectively. The highest chlorophyll a (0.678 ± 0.005 pg/cell), total carotenoids (0.18 ± 0.003 pg/cell), phycoerythrin (3.88 ± 0.003 pg/cell), and proteins (14.58 ± 0.35 pg/cell) contents were observed with F/2 medium. Cultivating Porphyridium sp. in both F/2 and Hemerick media yielded similar levels of starch accumulation. The Hemerick medium has proven to be the most suitable for the production of lipids (2.23% DW) and exopolysaccharides (5.41 ± 0.56 pg/cell).

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增强卟啉藻新菌株的生长和代谢物产量
微藻类能够产生多种代谢产物,具有显著的生物活性,在各种工业应用中大有可为。然而,这些光合微生物的分类多样性尚未得到深入研究。利用 18S rRNA 编码基因,最近发现的一株来自突尼斯海岸(马赫迪亚省)的菌株被鉴定为 Porphyridium 属的成员。研究人员对 Porphyridium sp.对不同培养基(Pm、F/2 和 Hemerick)的生长反应和代谢物积累进行了为期 52 天的调查。Pm 培养基的生物量产量最高(2 × 107 个细胞/毫升)。表观生长率(µ)和倍增时间(Dt)分别约为 0.081 天-1 和 12.34 天。叶绿素 a(0.678 ± 0.005 pg/细胞)、总类胡萝卜素(0.18 ± 0.003 pg/细胞)、植物红素(3.88 ± 0.003 pg/细胞)和蛋白质(14.58 ± 0.35 pg/细胞)含量在 F/2 培养基中最高。在 F/2 和 Hemerick 培养基中培养卟啉藻,淀粉积累水平相似。事实证明,Hemerick 培养基最适合生产脂质(2.23% DW)和外多糖(5.41 ± 0.56 pg/细胞)。
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来源期刊
Bioengineered
Bioengineered BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
8.20
自引率
28.60%
发文量
1114
审稿时长
17 weeks
期刊介绍: Bioengineered provides a platform for publishing high quality research on any aspect of genetic engineering which involves the generation of recombinant strains (both prokaryote and eukaryote) for beneficial applications in food, medicine, industry, environment and bio-defense.
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