Yingchun Ma, Bowen Li, Dejia Yang, Shuguang Wang, Lixia Yu, Hui Zhan, Juan Li
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引用次数: 0
Abstract
High-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that the commercial DNA extraction kit method was the most effective and comprehensive approach for extracting genomic DNA from shoots of four Dendrocalamus species. Furthermore, this study provided valuable insights into optimizing techniques for extracting genomic DNA in other bamboo species.
高质量的基因组 DNA 提取是植物基因克隆和表达研究的基础。因此，本研究评估了从四种石斛嫩枝中提取基因组 DNA 的几种方法，以确定最佳技术。基因组 DNA 的提取采用了三种不同的方法：商业 DNA 提取试剂盒法、改良十六烷基三甲基溴化铵法和十二烷基硫酸钠法。比较这些方法时采用了成员函数分析法。结果表明，商业 DNA 提取试剂盒法是从四种 Dendrocalamus 树种的嫩枝中提取基因组 DNA 的最有效、最全面的方法。此外，这项研究还为优化其他竹种的基因组 DNA 提取技术提供了宝贵的启示。
期刊介绍：
BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies.
Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers:
Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions
Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices
Surveys of technical approaches related to broad fields of research
Reviews discussing advancements in techniques and methods related to broad fields of research
Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.
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