Line-field confocal optical coherence tomography for in vivo visualization of morphological characteristics of squamous cell carcinoma in a murine model

IF 2.2 3区医学 Q2 DERMATOLOGY Lasers in Surgery and Medicine Pub Date : 2023-12-21 DOI:10.1002/lsm.23750

Uffe H. Olesen PhD, Camilla Foged MD, Kevin Jacobsen MD, Vinzent K. Ortner PhD, Gabriella Fredman MD, Uwe Paasch PhD, Merete Haedersdal PhD, DMSc

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Abstract

Objectives

Non-invasive imaging with line-field confocal optical coherence tomography (LC-OCT) can support the diagnosis of squamous cell carcinoma (SCC) through visualization of morphological characteristics specific to skin cancer. We aimed to visualize prominent morphological characteristics of SCC using LC-OCT in a well-established murine SCC model.

Materials and Methods

Nine hairless mice were exposed to ultraviolet radiation three times weekly for 9 months to induce SCC development. Visible SCC tumors (n = 9) were imaged with LC-OCT and the presence of 10 well-described morphological characteristics of SCC were evaluated in the scans by two physicians with adjudication by a third.

Results

Overall, murine morphological characteristics resembled corresponding features previously reported in human SCCs. Interrupted dermal-epidermal junction occurred in 100% of tumors. In epidermis, the most frequently observed characteristics were severe epidermal dysplasia (100%) and tumor budding (89%). Common dermal characteristics included broad strands (100%) and collagen alterations (78%).

Conclusion

LC-OCT imaging can be used to non-invasively visualize morphological characteristics specific to SCC in an in vivo preclinical model.

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线场共聚焦光学相干断层成像用于在鼠模型中活体观察鳞状细胞癌的形态特征。

目的：线场共聚焦光学相干断层扫描（LC-OCT）的无创成像可以通过观察皮肤癌特有的形态特征来支持鳞状细胞癌（SCC）的诊断。我们的目的是在一个成熟的小鼠鳞状细胞癌模型中使用 LC-OCT 观察鳞状细胞癌的突出形态特征：九只无毛小鼠每周暴露于紫外线辐射三次，持续 9 个月，以诱导 SCC 的发展。用 LC-OCT 对可见的 SCC 肿瘤（n = 9）进行成像，并由两名医生对扫描结果中是否存在 10 种明确描述的 SCC 形态特征进行评估，由第三名医生进行裁定：结果：总体而言，小鼠的形态特征与之前报道的人类 SCC 的相应特征相似。100%的肿瘤都出现了真皮-表皮交界中断。在表皮中，最常观察到的特征是严重的表皮发育不良（100%）和肿瘤出芽（89%）。常见的真皮特征包括宽股（100%）和胶原改变（78%）：结论：LC-OCT成像可用于在体内临床前模型中非侵入性地观察SCC特有的形态特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lasers in Surgery and Medicine 医学-外科

CiteScore

5.40

自引率

12.50%

发文量

119

审稿时长

1 months

期刊介绍： Lasers in Surgery and Medicine publishes the highest quality research and clinical manuscripts in areas relating to the use of lasers in medicine and biology. The journal publishes basic and clinical studies on the therapeutic and diagnostic use of lasers in all the surgical and medical specialties. Contributions regarding clinical trials, new therapeutic techniques or instrumentation, laser biophysics and bioengineering, photobiology and photochemistry, outcomes research, cost-effectiveness, and other aspects of biomedicine are welcome. Using a process of rigorous yet rapid review of submitted manuscripts, findings of high scientific and medical interest are published with a minimum delay.