Determination and verification of reference intervals of serum immunoglobulin G at birth.

IF 2.1 4区医学 Q3 MEDICAL LABORATORY TECHNOLOGY Annals of Clinical Biochemistry Pub Date : 2024-07-01 Epub Date: 2023-12-28 DOI:10.1177/00045632231225326

Toshihiko Ikuta, Sota Iwatani, Seiji Yoshimoto

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Abstract

Background: To accurately assess hypogammaglobulinemia at birth, it is essential to determine the reference intervals of serum immunoglobulin (IgG) levels in newborns. In the present study, we determined the gestational age (GA)-/birth weight (BW)-dependent percentile-based reference intervals of serum IgG levels and converted them into simple formulas for practical use.

Methods: Serum IgG levels were measured in cord blood from 2902 newborns delivered at 22 to 41 weeks of GA or 264 to 4642 g of BW after exclusion of those with congenital disorders. Linear regression analysis was used to correlate GA and UC-IgG levels and BW and UC-IgG levels. After calculation of the percentile values of UC-IgG levels for each GA or BW, the distributions were approximated by the least-squares method. Fitness was evaluated by the coefficient of determination (R²).

Results: Significant positive correlations were found both between GA and UC-IgG levels (r_s = 0.790, P < 0.001) and BW and UC-IgG levels (r_s = 0.626, P < 0.001). The distribution of the 5%ile of UC-IgG levels (Y) by GA or BW (X) was approximated as a straight line (Y = 37.5 *X - 775.8; Y = 0.161 *X + 95.34, respectively). The fitness was stronger in the GA-derived formula than the BW-derived formula (R² = 0.973 vs 0.913).

Conclusions: We established GA-/BW-dependent reference percentile-based intervals for serum IgG levels using cord blood from 2902 newborns without congenital disorders. Using GA-dependent reference intervals may be useful for assessing hypogammaglobulinemia at birth.

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出生时血清免疫球蛋白 G 参考区间的测定与验证。

背景：为了准确评估出生时的低丙种球蛋白血症，必须确定新生儿血清免疫球蛋白（IgG）水平的参考区间。在本研究中，我们确定了基于胎龄（GA）/体重（BW）百分位数的血清 IgG 水平参考区间，并将其转换成简单的公式供实际使用：在排除先天性疾病的新生儿后，对 2902 名胎龄在 22 至 41 周或体重在 264 至 4642 克的新生儿的脐带血中的血清 IgG 水平进行了测定。采用线性回归分析将GA与UC-IgG水平、体重与UC-IgG水平联系起来。在计算出每种 GA 或体重的 UC-IgG 水平百分位值后，用最小二乘法对其分布进行近似处理。用判定系数（R2）评估适配性：GA和UC-IgG水平（rs = 0.790，p < 0.001）以及BW和UC-IgG水平（rs = 0.626，p < 0.001）之间均存在显著的正相关。UC-IgG水平（Y）的5%ile分布按GA或体重（X）近似为一条直线（分别为Y = 55.2 * X - 1163.0; Y = 0.244 * X + 247.9）。GA公式比BW公式的适配性更强（R2 = 0.973 vs. 0.766）：我们利用 2902 名无先天性疾病的新生儿的脐带血，建立了基于 GA/BW 的血清 IgG 水平参考百分位数区间。使用 GA 依赖性参考区间可能有助于评估出生时的低丙种球蛋白血症。

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来源期刊

Annals of Clinical Biochemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

5.20

自引率

4.50%

发文量

期刊介绍： Annals of Clinical Biochemistry is the fully peer reviewed international journal of the Association for Clinical Biochemistry and Laboratory Medicine. Annals of Clinical Biochemistry accepts papers that contribute to knowledge in all fields of laboratory medicine, especially those pertaining to the understanding, diagnosis and treatment of human disease. It publishes papers on clinical biochemistry, clinical audit, metabolic medicine, immunology, genetics, biotechnology, haematology, microbiology, computing and management where they have both biochemical and clinical relevance. Papers describing evaluation or implementation of commercial reagent kits or the performance of new analysers require substantial original information. Unless of exceptional interest and novelty, studies dealing with the redox status in various diseases are not generally considered within the journal''s scope. Studies documenting the association of single nucleotide polymorphisms (SNPs) with particular phenotypes will not normally be considered, given the greater strength of genome wide association studies (GWAS). Research undertaken in non-human animals will not be considered for publication in the Annals. Annals of Clinical Biochemistry is also the official journal of NVKC (de Nederlandse Vereniging voor Klinische Chemie) and JSCC (Japan Society of Clinical Chemistry).