Nitric oxide and ion channels mediate L-cysteine-induced inhibition of colonic smooth muscle contraction.

IF 1.8 3区 生物学 Q4 CELL BIOLOGY Journal of Muscle Research and Cell Motility Pub Date : 2024-03-01 Epub Date: 2023-12-23 DOI:10.1007/s10974-023-09664-2
{"title":"Nitric oxide and ion channels mediate L-cysteine-induced inhibition of colonic smooth muscle contraction.","authors":"Xiaojing Quan, Min Zhang, Zhaojun Qiao, Xuan Kou, Qiong Xue, Jinhai Wang, Lu Li","doi":"10.1007/s10974-023-09664-2","DOIUrl":null,"url":null,"abstract":"<p><p>Previous studies have suggested that L-cysteine regulates gut motility through hydrogen sulfide. However, the mechanisms involved in the L-cysteine-induced response have not been extensively studied. This study aimed to investigate the underlying mechanisms of action of L-cysteine on spontaneous contraction of rat colon. Longitudinal and circular muscle strips from rat middle colon were prepared to measure the spontaneous contractile activities of colon in an organ bath system. Whole-cell voltage-clamp techniques were applied to record the currents of L-type voltage-dependent Ca<sup>2+</sup> channels (VDCCs) and voltage-gated K<sup>+</sup> channels (Kv) in isolated smooth muscle cells (SMCs) from colon. L-cysteine inhibited the spontaneous contraction of longitudinal and circular muscle strips from the rat colon in a concentration-dependent manner. The inhibition induced by L-cysteine was significantly decreased by inhibitors of H<sub>2</sub>S synthesis (p < 0.05). Furthermore, the suppression induced by L-cysteine was partially attenuated by tetrodotoxin, L-NNA and glibenclamide (p < 0.05). Whole-cell voltage-clamp recordings showed that L-cysteine caused a remarkable reduction in the peak currents of VDCCs and significantly increased the membrane currents of Kv channels in isolated SMCs (p < 0.05). We concluded that L-cysteine inhibits the contractile activities of smooth muscle strips from the rat colon. The relaxation in response to L-cysteine may be in part mediated by a nitrergic pathway and by inhibiting the VDCCs in combination with a direct activation of the K<sub>V</sub> channels and K<sub>ATP</sub> channels.</p>","PeriodicalId":16422,"journal":{"name":"Journal of Muscle Research and Cell Motility","volume":" ","pages":"11-20"},"PeriodicalIF":1.8000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Muscle Research and Cell Motility","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10974-023-09664-2","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/23 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Previous studies have suggested that L-cysteine regulates gut motility through hydrogen sulfide. However, the mechanisms involved in the L-cysteine-induced response have not been extensively studied. This study aimed to investigate the underlying mechanisms of action of L-cysteine on spontaneous contraction of rat colon. Longitudinal and circular muscle strips from rat middle colon were prepared to measure the spontaneous contractile activities of colon in an organ bath system. Whole-cell voltage-clamp techniques were applied to record the currents of L-type voltage-dependent Ca2+ channels (VDCCs) and voltage-gated K+ channels (Kv) in isolated smooth muscle cells (SMCs) from colon. L-cysteine inhibited the spontaneous contraction of longitudinal and circular muscle strips from the rat colon in a concentration-dependent manner. The inhibition induced by L-cysteine was significantly decreased by inhibitors of H2S synthesis (p < 0.05). Furthermore, the suppression induced by L-cysteine was partially attenuated by tetrodotoxin, L-NNA and glibenclamide (p < 0.05). Whole-cell voltage-clamp recordings showed that L-cysteine caused a remarkable reduction in the peak currents of VDCCs and significantly increased the membrane currents of Kv channels in isolated SMCs (p < 0.05). We concluded that L-cysteine inhibits the contractile activities of smooth muscle strips from the rat colon. The relaxation in response to L-cysteine may be in part mediated by a nitrergic pathway and by inhibiting the VDCCs in combination with a direct activation of the KV channels and KATP channels.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
一氧化氮和离子通道介导 L-半胱氨酸诱导的结肠平滑肌收缩抑制。
以往的研究表明,L-半胱氨酸可通过硫化氢调节肠道蠕动。然而,L-半胱氨酸诱导反应的机制尚未得到广泛研究。本研究旨在探究 L-半胱氨酸对大鼠结肠自发收缩的潜在作用机制。研究人员制备了大鼠结肠中段的纵肌条和环肌条,在器官浴系统中测量结肠的自发收缩活动。应用全细胞电压钳技术记录了大肠离体平滑肌细胞(SMCs)中 L 型电压依赖性 Ca2+ 通道(VDCCs)和电压门控 K+ 通道(Kv)的电流。L-半胱氨酸以浓度依赖性方式抑制大鼠结肠纵肌和环肌条带的自发收缩。H2S 合成抑制剂(p V 通道和 KATP 通道)可显著降低 L-半胱氨酸的抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
相关文献
Interleukin-1β-induced, nitric oxide-dependent and -independent inhibition of vascular smooth muscle contraction
IF 4.2 3区 医学European journal of pharmacologyPub Date : 1997-07-09 DOI: 10.1016/S0014-2999(97)00164-7
Satoko Takizawa, Hiroshi Ozaki, Hideaki Karaki
Calcium and protein kinase C mediate high-glucose-induced inhibition of inducible nitric oxide synthase in vascular smooth muscle cells.
IF 8.3 1区 医学HypertensionPub Date : 1998-01-01 DOI: 10.1161/01.hyp.31.1.289
R Muniyappa, P R Srinivas, J L Ram, M F Walsh, J R Sowers
Multiple potassium channels mediate nitric oxide-induced inhibition of rat vascular smooth muscle cell proliferation
IF 3.9 2区 生物学Nitric oxide : biology and chemistryPub Date : 2005-09-01 DOI: 10.1016/j.niox.2005.05.010
Renata S.A. Costa, Jamil Assreuy
来源期刊
CiteScore
6.20
自引率
0.00%
发文量
21
审稿时长
>12 weeks
期刊介绍: The Journal of Muscle Research and Cell Motility has as its main aim the publication of original research which bears on either the excitation and contraction of muscle, the analysis of any one of the processes involved therein, the processes underlying contractility and motility of animal and plant cells, the toxicology and pharmacology related to contractility, or the formation, dynamics and turnover of contractile structures in muscle and non-muscle cells. Studies describing the impact of pathogenic mutations in genes encoding components of contractile structures in humans or animals are welcome, provided they offer mechanistic insight into the disease process or the underlying gene function. The policy of the Journal is to encourage any form of novel practical study whatever its specialist interest, as long as it falls within this broad field. Theoretical essays are welcome provided that they are concise and suggest practical ways in which they may be tested. Manuscripts reporting new mutations in known disease genes without validation and mechanistic insight will not be considered. It is the policy of the journal that cells lines, hybridomas and DNA clones should be made available by the developers to any qualified investigator. Submission of a manuscript for publication constitutes an agreement of the authors to abide by this principle.
期刊最新文献
Plasticity in leukocyte migration during haematopoiesis and inflammation. Fluorescence lifetime imaging microscopy of endogenous fluorophores in health and disease. Differential impact of substrates on myosin heavy and light chain expression in human stem cell-derived cardiomyocytes at single-cell level. Progress and prospects in antisense oligonucleotide-mediated exon skipping therapies for Duchenne muscular dystrophy. The role of MEGF10 in myoblast fusion and hypertrophic response to overload of skeletal muscle.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1