Preliminary Trial of Male to Female Sex Reversal by 17β-Estradiol in Combination with Trilostane in Spotted Scat (Scatophagus argus)

IF 2.1 3区 农林科学 Q2 FISHERIES Fishes Pub Date : 2023-12-19 DOI:10.3390/fishes9010001
Zheng-Ting Jiang, Gang Shi, Dongneng Jiang, Yu Li, Yuang-Qing Huang, Hong-Juan Shi, Guang-Li Li
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Abstract

The spotted scat (Scatophagus argus) is an important ornamental species with sexually biased ornamental values that favor males. Therefore, it makes sense to breed mono-male fingerlings as ornamental fish. The spotted scat has an XX/XY sex determination system; therefore, the first step in producing genetically all-male offspring should be the induction of a fertile sex-reversed XY pseudo-female, which would then be mated with a normal XY male to produce a YY super-male. However, the XY pseudo-female produced by estradiol (E2) treatment failed due to ovarian malformations. Here, male to female sex reversal was induced in spotted scat through a combination of E2 and an androgen inhibitor (trilostane, TR). Spotted scat fingerlings of approximately 2.8–3.5 cm were fed a diet containing both E2 (300 µg/g) and TR (300 µg/g). The fish were treated for 90 days and then fed a normal diet until they reached one year of age, when they were sampled. Twenty-eight treated XY individuals were identified using sex-linked markers. According to their gonadal histological characteristics, these treated XY fish could be divided into three groups: males with testes (n = 21), intersex individuals with ovaries–testes (n = 3), and fully sex-reversed individuals with ovaries (n = 4). All treated XX fish (n = 8) developed into normal females. There were no obvious abnormalities in the ovaries of the XY sex-reversed fish compared to the treated XX fish. Serum 11-ketotestosterone (11-KT) levels were significantly lower in XY-reversed females and treated XX females than in XY males. Compared with XY male fish, the expression of female (42sp50, foxl2, figla, zar1, and zp2) and male (dmrt1, gsdf, amh and cyp11b2) biased genes was up- and down-regulated, respectively, in the gonads of XY-completely sex-reversed fish. Immunohistochemical results indicate that 42Sp50 was expressed in oocytes of XY-partially and completely sex-reversed fish, while strong Gsdf signals were mainly detectable in testicular somatic cells of XY-partially sex-reversed fish and XY male fish. XY females were successfully produced by the combined treatment of E2 and TR in the spotted scat. Their fertility will be tested in the future.
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通过 17β 雌二醇与 Trilostane 组合对斑鼬粪便进行雄性逆转为雌性的初步试验
斑点叉尾鮰(Scatophagus argus)是一种重要的观赏鱼种,其观赏价值在性别上偏向雄性。因此,培育单雄幼鱼作为观赏鱼是有意义的。斑点叉尾鮰具有 XX/XY 性别决定系统;因此,培育全雄性基因后代的第一步应该是诱导可育的 XY 性别逆转假雌鱼,然后将其与正常的 XY 雄性交配,培育出 YY 超级雄鱼。然而,通过雌二醇(E2)治疗产生的XY假雌性因卵巢畸形而失败。在这里,通过 E2 和雄激素抑制剂(曲洛斯坦,TR)的组合,诱导了斑鳞鱼雄雌性别的逆转。用含有 E2(300 微克/克)和 TR(300 微克/克)的饲料喂养约 2.8-3.5 厘米的斑鳞指环鱼。对这些鱼进行 90 天的处理,然后喂食正常食物,直到它们长到一岁时采样。利用性连锁标记鉴定了 28 条经过处理的 XY 鱼。根据其性腺组织学特征,这些经过处理的 XY 鱼可分为三组:有睾丸的雄鱼(n = 21)、有卵巢-睾丸的双性鱼(n = 3)和有卵巢的完全性别逆转鱼(n = 4)。所有经过处理的 XX 鱼(n = 8)都发育成了正常的雌鱼。与处理过的XX鱼相比,XY性别逆转鱼的卵巢没有明显异常。XY性别逆转雌鱼和经处理的XX雌鱼的血清11-酮睾酮(11-KT)水平明显低于XY雄鱼。与 XY 雄性鱼类相比,雌性(42sp50、foxl2、figla、zar1 和 zp2)和雄性(dmrt1、gsdf、amh 和 cyp11b2)偏性基因在 XY 完全性别逆转鱼类性腺中的表达分别呈上调和下调趋势。免疫组化结果表明,42Sp50在XY部分性逆转鱼和完全性逆转鱼的卵母细胞中均有表达,而Gsdf的强信号主要在XY部分性逆转鱼和XY雄鱼的睾丸体细胞中可检测到。通过对斑点叉尾鱼粪中的 E2 和 TR 进行联合处理,成功培育出了 XY 雌鱼。今后将对它们的生育能力进行测试。
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Fishes
Fishes Multiple-
CiteScore
1.90
自引率
8.70%
发文量
311
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