A. V. Boyko, E. A. Mikheeva, T. L. Zakharova, N. A. Osina
{"title":"Detection of Cholera Toxin in Culture Medium by Dot-Immunoassay on Dipsticks","authors":"A. V. Boyko, E. A. Mikheeva, T. L. Zakharova, N. A. Osina","doi":"10.1134/S0003683823080045","DOIUrl":null,"url":null,"abstract":"<div><p>An instrument-free method for detecting the ability of <i>Vibrio cholerae</i> strains to produce toxin has been developed. It is based on dot-ELISA performed on dipstiks. Dipsticks require 100 times less monoclonal antibodies than microplate ELISA.</p></div>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 8","pages":"1113 - 1117"},"PeriodicalIF":1.0000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683823080045","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
An instrument-free method for detecting the ability of Vibrio cholerae strains to produce toxin has been developed. It is based on dot-ELISA performed on dipstiks. Dipsticks require 100 times less monoclonal antibodies than microplate ELISA.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
