From sequence to function and back – High-throughput sequence-function mapping in synthetic biology

IF 3.4 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Current Opinion in Systems Biology Pub Date : 2023-12-13 DOI:10.1016/j.coisb.2023.100499
Simon Höllerer , Charlotte Desczyk , Ricardo Farrera Muro , Markus Jeschek
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Abstract

How does genetic sequence give rise to biological function? Answering this question is key to our understanding of life and the construction of synthetic biosystems that fight disease, resource scarcity and climate change. Unfortunately, the virtually infinite number of possible sequences and limitations in their functional characterization limit our current understanding of sequence-function relationships. To overcome this dilemma, several high-throughput methods to experimentally link sequences to corresponding functional properties have been developed recently. While all of these share the goal to collect sequence-function data at large scale, they differ significantly in their technical approach, functional readout and application scope. Herein, we highlight recent developments in the aspiring field of high-throughput sequence-function mapping providing a critical assessment of their potential in synthetic biology.

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从序列到功能再到序列--合成生物学中的高通量序列功能图谱
基因序列如何产生生物功能?回答这个问题是我们了解生命、构建合成生物系统以对抗疾病、资源匮乏和气候变化的关键。遗憾的是,可能存在的序列数量几乎无穷无尽,而其功能表征却存在局限性,这限制了我们目前对序列-功能关系的理解。为了克服这一困境,最近开发了几种高通量方法,通过实验将序列与相应的功能特性联系起来。虽然所有这些方法的共同目标都是大规模收集序列-功能数据,但它们在技术方法、功能读出和应用范围上有很大不同。在此,我们将重点介绍高通量序列-功能图谱这一令人向往的领域的最新进展,并对其在合成生物学中的潜力进行批判性评估。
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来源期刊
Current Opinion in Systems Biology
Current Opinion in Systems Biology Mathematics-Applied Mathematics
CiteScore
7.10
自引率
2.70%
发文量
20
期刊介绍: Current Opinion in Systems Biology is a new systematic review journal that aims to provide specialists with a unique and educational platform to keep up-to-date with the expanding volume of information published in the field of Systems Biology. It publishes polished, concise and timely systematic reviews and opinion articles. In addition to describing recent trends, the authors are encouraged to give their subjective opinion on the topics discussed. As this is such a broad discipline, we have determined themed sections each of which is reviewed once a year. The following areas will be covered by Current Opinion in Systems Biology: -Genomics and Epigenomics -Gene Regulation -Metabolic Networks -Cancer and Systemic Diseases -Mathematical Modelling -Big Data Acquisition and Analysis -Systems Pharmacology and Physiology -Synthetic Biology -Stem Cells, Development, and Differentiation -Systems Biology of Mold Organisms -Systems Immunology and Host-Pathogen Interaction -Systems Ecology and Evolution
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From regulation of cell fate decisions towards patient-specific treatments, insights from mechanistic models of signalling pathways Editorial overview: Systems biology of ecological interactions across scales A critical review of multiscale modeling for predictive understanding of cancer cell metabolism Network modeling approaches for metabolic diseases and diabetes Contents
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