Facile, sensitive and reagent-saving smartphone-based digital image colorimetric assay of captopril tablets enabled by long-pathlength RGB acquisition

IF 1.1 Q4 PHARMACOLOGY & PHARMACY Pharmacia Pub Date : 2023-12-11 DOI:10.3897/pharmacia.70.e114927

Thana Thanayutsiri, Thapakorn Charoenying, Prasopchai Patrojanasophon, Boonnada Pamornpathomkul, P. Opanasopit, T. Ngawhirunpat, T. Rojanarata, Prasopchai Patrojanasophon

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Abstract

A new assay of captopril (CTP) tablets was developed based on digital image colorimetry using Ellman’s reagent. For the first time, a facile technique of increasing the analytical path was applied in this work to enhance the sensitivity. For this purpose, the reaction solutions were photographed using a smartphone while they were contained in two 1-cm pathlength cuvettes which were placed side by side. The Red-Green-Blue (RGB) in term of [B/(R+G+B)] was used to plot a standard curve. Compared to using a single cuvette, double cuvettes resulted in more precise analytical signals and better linearity (r2 of 0.9992). Additionally, CTP could be analyzed at low concentrations (2.5–25 µM) with LOD of 0.70 µM and LOQ of 2.13 µM, thus lowering the reagent consumption. The assay was proven to be valid, and it was greener, faster, and more affordable than the pharmacopeial chromatographic method, thereby suitable for pharmaceutical quality control.

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基于智能手机的卡托普利片剂数字图像比色法，采用长波长 RGB 采集技术，简便、灵敏且节省试剂

基于数字图像比色法，使用埃尔曼试剂开发了卡托普利片（CTP）的新型检测方法。在这项工作中，首次采用了增加分析路径的简便技术来提高灵敏度。为此，使用智能手机对并排放置的两个 1 厘米路径长度的比色皿中的反应溶液进行拍照。以 [B/(R+G+B)] 为单位的红绿蓝 (RGB) 被用来绘制标准曲线。与使用单比色皿相比，双比色皿的分析信号更精确，线性度更好（r2 为 0.9992）。此外，CTP 可在低浓度（2.5-25 µM）下进行分析，LOD 为 0.70 µM，LOQ 为 2.13 µM，从而降低了试剂消耗量。与药典色谱法相比，该检测方法更环保、更快速、更经济，因此适用于药品质量控制。

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