Effects of straw incorporation on biological nitrogen fixation under continuous and intermittent flooding irrigation in a rice cropping system

IF 7.3 2区农林科学 Q1 SOIL SCIENCE Pedosphere Pub Date : 2023-12-19 DOI:10.1016/j.pedsph.2023.12.007

Yanhui ZHANG , Jing WANG , Qi LIU , Haihou WANG , Tianlong HU , Hui WANG , Zhe CHEN , Liangzuo SHU , Elrys S. AHMED , Zubin XIE

{"title":"Effects of straw incorporation on biological nitrogen fixation under continuous and intermittent flooding irrigation in a rice cropping system","authors":"Yanhui ZHANG , Jing WANG , Qi LIU , Haihou WANG , Tianlong HU , Hui WANG , Zhe CHEN , Liangzuo SHU , Elrys S. AHMED , Zubin XIE","doi":"10.1016/j.pedsph.2023.12.007","DOIUrl":null,"url":null,"abstract":"<div><div>Both straw incorporation and irrigation practices affect biological nitrogen (N) fixation (BNF), but it is still unclear how straw incorporation impacts BNF under continuous (CFI) or intermittent (IFI) flooding irrigation in a rice cropping system. A 15N2-labeling chamber system was placed in a rice field to evaluate BNF with straw incorporation under CFI or IFI for 90 d. The nifH (gene encoding the nitrogenase reductase subunit) DNA and cDNA in soil were amplified using real-time quantitative polymerase chain reaction, and high-throughput sequencing was applied to the nifH gene. The total fixed N in the straw incorporation treatment was 14.3 kg ha−1 under CFI, being 116% higher than that under IFI (6.62 kg ha−1). Straw incorporation and CFI showed significant interactive effects on the total fixed N and abundances of nifH DNA and cDNA. The increase in BNF was mainly due to the increase in the abundances of heterotrophic diazotrophs such as Desulfovibrio, Azonexus, and Azotobacter. These results indicated that straw incorporation stimulated BNF under CFI relative to IFI, which might ultimately lead to a rapid enhancement of soil fertility.</div></div>","PeriodicalId":49709,"journal":{"name":"Pedosphere","volume":"35 2","pages":"Pages 364-372"},"PeriodicalIF":7.3000,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pedosphere","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1002016023001303","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SOIL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Both straw incorporation and irrigation practices affect biological nitrogen (N) fixation (BNF), but it is still unclear how straw incorporation impacts BNF under continuous (CFI) or intermittent (IFI) flooding irrigation in a rice cropping system. A ¹⁵N₂-labeling chamber system was placed in a rice field to evaluate BNF with straw incorporation under CFI or IFI for 90 d. The nifH (gene encoding the nitrogenase reductase subunit) DNA and cDNA in soil were amplified using real-time quantitative polymerase chain reaction, and high-throughput sequencing was applied to the nifH gene. The total fixed N in the straw incorporation treatment was 14.3 kg ha⁻¹ under CFI, being 116% higher than that under IFI (6.62 kg ha⁻¹). Straw incorporation and CFI showed significant interactive effects on the total fixed N and abundances of nifH DNA and cDNA. The increase in BNF was mainly due to the increase in the abundances of heterotrophic diazotrophs such as Desulfovibrio, Azonexus, and Azotobacter. These results indicated that straw incorporation stimulated BNF under CFI relative to IFI, which might ultimately lead to a rapid enhancement of soil fertility.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

水稻种植系统连续和间歇漫灌条件下秸秆添加对生物固氮的影响

秸秆掺入和灌溉方式都影响生物固氮（BNF），但目前尚不清楚秸秆掺入如何影响水稻种植系统中连续（CFI）或间歇（IFI）漫灌条件下的生物固氮（BNF）。将15n2标记室系统置于稻田中，在CFI或IFI条件下对秸秆还原性进行90 d的评价。利用实时定量聚合酶链反应扩增土壤中nifH（编码氮酶还原酶亚基的基因）DNA和cDNA，并对nifH基因进行高通量测序。秸秆还田处理的总固定氮量为14.3 kg ha - 1，比IFI处理（6.62 kg ha - 1）提高了116%。秸秆还田和CFI对固定氮总量、nifH DNA和cDNA丰度均有显著的交互作用。BNF的增加主要是由于Desulfovibrio、Azonexus和Azotobacter等异养重氮营养菌丰度的增加。这些结果表明，相对于IFI， CFI下秸秆还田促进了土壤BNF，最终可能导致土壤肥力的快速提高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Pedosphere 环境科学-土壤科学

CiteScore

11.70

自引率

1.80%

发文量

147

审稿时长

5.0 months

期刊介绍： PEDOSPHERE—a peer-reviewed international journal published bimonthly in English—welcomes submissions from scientists around the world under a broad scope of topics relevant to timely, high quality original research findings, especially up-to-date achievements and advances in the entire field of soil science studies dealing with environmental science, ecology, agriculture, bioscience, geoscience, forestry, etc. It publishes mainly original research articles as well as some reviews, mini reviews, short communications and special issues.