Analysis of bioactive hispidulin: an anticancer flavone of Clerodendrum philippinum

IF 1.1 4区 化学 Q4 CHEMISTRY, ANALYTICAL Jpc-journal of Planar Chromatography-modern Tlc Pub Date : 2023-12-22 DOI:10.1007/s00764-023-00267-8
Kedar Kumar Rout, Mihir Kumar Kar, Prakash Chandra Agarwal, Sarat Kumar Dash
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Abstract

Hispidulin has been isolated from the leaves of Clerodendrum philippinum and identified with the help of physical and spectroscopic data. A high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for its accurate estimation in different plant parts of C. philippinum. Thin-layer chromatographic (TLC) analysis was performed on HPTLC plates by use of a ternary mobile phase consisting of chloroform‒methanol‒formic acid (9:1:0.1, V/V). It was quantified at the wavelength of its maximum absorbance at 267 nm, with a linear response in the range of 100‒500 ng per spot. The method was validated according to the International Council for Harmonisation (ICH) guidelines. The developed method was found to be more highly sensitive than the previously reported methods and gives consistent results when applied for the postchromatographic estimation of hispidulin. The developed method is very accurate, precise, and has been successfully applied for the analysis of hispidulin in C. philippinum, and also can be used for its quality control and standardization.

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分析具有生物活性的糙硬毛蕊花素:一种抗癌黄酮
通过物理和光谱数据,从荚蒾叶中分离并鉴定了糙叶素。开发并验证了一种高效薄层色谱(HPTLC)方法,用于准确估算鳞叶甘草不同植物部位中的鳞叶甘草次苷含量。采用氯仿-甲醇-甲酸(9:1:0.1, V/V)三元流动相在 HPTLC 板上进行薄层色谱(TLC)分析。其最大吸光度波长为 267 nm,定量范围为 100-500 ng/点。该方法根据国际协调理事会(ICH)指南进行了验证。与之前报道的方法相比,所开发的方法灵敏度更高,且在色谱后估算糙皮苷含量时结果一致。所开发的方法非常准确、精确,已成功用于分析菲利宾鱼中的糙皮素,也可用于其质量控制和标准化。
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来源期刊
CiteScore
2.20
自引率
18.80%
发文量
66
审稿时长
>12 weeks
期刊介绍: JPC - Journal of Planar Chromatography - Modern TLC is an international journal devoted exclusively to the publication of research papers on analytical and preparative planar chromatography. The journal covers all fields of planar chromatography, on all kinds of stationary phase (paper, layer, gel) and with various modes of migration of the mobile phase (capillary action or forced flow).
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