Quantifying plasma dacarbazine levels in advanced melanoma patients: a liquid chromatography-tandem mass spectrometry performance analysis.

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2023-12-01 Epub Date: 2024-01-24 DOI:10.1080/00365513.2023.2297356
Wenhan Cheng, John Liu, Bryan Jackson
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Abstract

The aim of this study was to develop a robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying dacarbazine levels in the plasma of advanced melanoma patients, followed by an assessment of its analytical capabilities. The research encompassed the design of a high-performance liquid chromatography (HPLC) system, with the quantitative analysis performed using the multiple reaction monitoring (MRM) techniques and specific ion transition: 181.0 > 152.5 for dacarbazine and 187.1 > 158.6 for the internal standard (IS), dacarbazine-D6. The validation of the method involved an evaluation of parameters including linearity, detection limit, precision, and accuracy. Notably, the linear range extended from 10 to 1,000 µg/L for dacarbazine, and the method exhibited a detection limit of 10 µg/L. The method's precision, indicated by within-run and between-run coefficients of variation (CV), both being ≤4.2% and ≤8.3%, respectively. Furthermore, the accuracy of measurements, ranging from 86.1% to 99.4%, underscored the method's reliability. In clinical application, the dacarbazine levels of healthy control (n = 20) were 0.6 ± 0.02 μg/L; 770.9 ± 203.2 μg/mL in early-stage-melanoma patients (n = 22), and 588.7 ± 153.2 μg/mL in advanced melanoma patients (n = 25). The results serve as clinical evidence showing that long-term dacarbazine treatment affects the metabolism of dacarbazine.

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晚期黑色素瘤患者血浆达卡巴嗪水平的定量分析:液相色谱-串联质谱性能分析。
本研究旨在开发一种稳健的液相色谱-串联质谱(LC-MS/MS)方法,用于定量检测晚期黑色素瘤患者血浆中达卡巴嗪的水平,并对其分析能力进行评估。这项研究包括设计一个高效液相色谱(HPLC)系统,利用多反应监测(MRM)技术和特定离子转换进行定量分析:达卡巴嗪的离子转换率为 181.0 > 152.5,内标(IS)达卡巴嗪-D6 的离子转换率为 187.1 > 158.6。该方法的验证包括对线性、检测限、精密度和准确度等参数的评估。值得注意的是,达卡巴嗪的线性范围为 10 至 1,000 µg/L,方法的检测限为 10 µg/L。该方法的精密度(以运行内变异系数和运行间变异系数表示)分别为≤4.2%和≤8.3%。此外,测量的准确度从 86.1%到 99.4%不等,突出了该方法的可靠性。在临床应用中,健康对照组(20 人)的达卡巴嗪水平为 0.6 ± 0.02 μg/L;早期黑色素瘤患者(22 人)的达卡巴嗪水平为 770.9 ± 203.2 μg/mL;晚期黑色素瘤患者(25 人)的达卡巴嗪水平为 588.7 ± 153.2 μg/mL。这些结果作为临床证据表明,长期达卡巴嗪治疗会影响达卡巴嗪的代谢。
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来源期刊
CiteScore
3.50
自引率
4.80%
发文量
85
审稿时长
4-8 weeks
期刊介绍: The Scandinavian Journal of Clinical and Laboratory Investigation is an international scientific journal covering clinically oriented biochemical and physiological research. Since the launch of the journal in 1949, it has been a forum for international laboratory medicine, closely related to, and edited by, The Scandinavian Society for Clinical Chemistry. The journal contains peer-reviewed articles, editorials, invited reviews, and short technical notes, as well as several supplements each year. Supplements consist of monographs, and symposium and congress reports covering subjects within clinical chemistry and clinical physiology.
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