Functionalization of selenium nanoparticles with olive polyphenols - impact on toxicity and antioxidative activity.

IF 2.1 4区医学 Q3 PHARMACOLOGY & PHARMACY Acta Pharmaceutica Pub Date : 2023-12-26 Print Date: 2023-12-01 DOI:10.2478/acph-2023-0036

Emerik Galić, Kristina Radić, Nikolina Golub, Jakov Mlinar, Dubravka Vitali Čepo, Tomislav Vinković

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Abstract

Selenium nanoparticles (SeNPs) represent novel selenium (Se) formulation characterized by improved biocompatibility and a wider therapeutic range in comparison to inorganic Se. The aim of this work was to investigate the possibilities of functionalization of SeNPs with olive pomace extract (OPE), rich in health-promoting polyphenols, and to obtain innovative forms of nutraceuticals. Cytotoxic and antioxidative activities of four types of SeNPs (polyvinylpyrrolidone stabilized (PVP SeNPs), polysorbate stabilized (PS SeNPs), polyvinylpyrrolidone stabilized and functionalized using OPE (f PVP SeNPs) and polysorbate stabilized and functionalized using OPE (f PS SeNPs) were investigated. SeNPs showed lower toxicity on human hepatocellular carcinoma (HepG2) and human colorectal adenocarcinoma (Caco2) cells compared to selenite. Functionalization with polyphenols significantly improved their direct antiradical (f PVP SeNPs: 24.4 ± 1.84 and f PS SeNPs: 30.9 ± 2.47 mg TE/mmol Se) and reducing properties (f PVP SeNPs: 50 ± 3.16 and f PS SeNPs: 53.6 ± 3.22 mg GAE/mmol) compared to non-functionalized SeNPs. The significant impact of tested SeNPs on intracellular antioxidative mechanisms has been observed and it was dependent on both cell type and physico-chemical properties of SeNPs, indicating the complexity of involved mechanisms.

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硒纳米颗粒与橄榄多酚的功能化--对毒性和抗氧化活性的影响。

硒纳米粒子（SeNPs）是一种新型硒（Se）制剂，与无机硒相比，其生物相容性更好，治疗范围更广。这项工作的目的是研究用富含促进健康的多酚的橄榄渣提取物（OPE）对 SeNPs 进行功能化的可能性，并获得创新形式的营养保健品。研究了四种 SeNPs（聚乙烯吡咯烷酮稳定型 SeNPs、聚山梨醇酯稳定型 SeNPs、聚乙烯吡咯烷酮稳定型并使用 OPE 功能化的 SeNPs（f PVP SeNPs）和聚山梨醇酯稳定型并使用 OPE 功能化的 SeNPs（f PS SeNPs））的细胞毒性和抗氧化活性。与亚硒酸盐相比，SeNPs 对人类肝细胞癌（HepG2）和人类结直肠腺癌（Caco2）细胞的毒性较低。与未功能化的 SeNPs 相比，多酚功能化显著提高了其直接抗自由基（f PVP SeNPs：24.4 ± 1.84 和 f PS SeNPs：30.9 ± 2.47 mg TE/mmol Se）和还原性（f PVP SeNPs：50 ± 3.16 和 f PS SeNPs：53.6 ± 3.22 mg GAE/mmol）。已观察到测试的 SeNPs 对细胞内抗氧化机制产生了重大影响，而且这种影响取决于细胞类型和 SeNPs 的物理化学特性，这表明了相关机制的复杂性。

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来源期刊

Acta Pharmaceutica PHARMACOLOGY & PHARMACY-

CiteScore

5.20

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： AP is an international, multidisciplinary journal devoted to pharmaceutical and allied sciences and contains articles predominantly on core biomedical and health subjects. The aim of AP is to increase the impact of pharmaceutical research in academia, industry and laboratories. With strong emphasis on quality and originality, AP publishes reports from the discovery of a drug up to clinical practice. Topics covered are: analytics, biochemistry, biopharmaceutics, biotechnology, cell biology, cell cultures, clinical pharmacy, drug design, drug delivery, drug disposition, drug stability, gene technology, medicine (including diagnostics and therapy), medicinal chemistry, metabolism, molecular modeling, pharmacology (clinical and animal), peptide and protein chemistry, pharmacognosy, pharmacoepidemiology, pharmacoeconomics, pharmacodynamics and pharmacokinetics, protein design, radiopharmaceuticals, and toxicology.