Identification of AGO2 and PLEC genes polymorphisms in Hu sheep and their relationship with body size traits.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-12-27 DOI:10.1080/10495398.2023.2295926

Jia Liu, Wenxin Zheng, Weimin Wang, Xiaobin Yang, Yongliang Huang, Panpan Cui, Zongwu Ma, Xiwen Zeng, Rui Zhai, Xiuxiu Weng, Weiwei Wu, Xiaoxue Zhang

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Abstract

The body size traits are major traits in livestock, which intuitively displays the development of the animal's bones and muscles. This study used PCR amplification, Sanger sequencing, KASPar genotyping, and quantitative real-time reverse transcription PCR (qRT-PCR) to analyze the Single-nucleotide polymorphism and expression characteristics of Argonaute RISC catalytic component 2 (AGO2) and Plectin (PLEC) genes in Hu sheep. Two intron mutations were found in Hu sheep, which were AGO2 g.51700 A > C and PLEC g.23157 C > T, respectively. Through association analysis of two mutation sites and body size traits, it was found that AGO2 g.51700 A > C mainly affects the chest and cannon circumference of Hu sheep of while PLEC g.23157 C mainly affects body height and body length. The combined genotypes of AGO2 and PLEC genes with body size traits showed SNPs at the AGO2 g.51700 A > C and PLEC g.23157 C > T loci significantly improved the body size traits of Hu sheep. In addition, the AGO2 gene has the highest expression levels in the heart, rumen, and tail fat, and the PLEC gene is highly expressed in the heart. These two loci can provide new research ideas for improving the body size traits of Hu sheep.

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胡羊 AGO2 和 PLEC 基因多态性的鉴定及其与体型特征的关系

体型是家畜的主要性状，它直观地显示了家畜骨骼和肌肉的发育情况。本研究采用PCR扩增、Sanger测序、KASPar基因分型和实时反转录定量PCR（qRT-PCR）技术分析了胡羊Argonaute RISC催化元件2（AGO2）和Plectin（PLEC）基因的单核苷酸多态性和表达特征。在胡羊中发现了两个内含子突变，分别为AGO2 g.51700 A > C和PLEC g.23157 C > T。通过对两个突变位点与体型性状的关联分析，发现AGO2 g.51700 A > C主要影响胡羊的胸围和炮围，而PLEC g.23157 C主要影响体高和体长。AGO2和PLEC基因的组合基因型与体型性状的关系表明，AGO2 g.51700 A > C和PLEC g.23157 C > T位点上的SNP显著改善了胡羊的体型性状。此外，AGO2 基因在心脏、瘤胃和尾脂中的表达水平最高，PLEC 基因在心脏中的表达水平也很高。这两个基因位点可为改善胡羊体型性状提供新的研究思路。

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来源期刊

Animal Biotechnology 工程技术-奶制品与动物科学

CiteScore

2.90

自引率

5.40%

发文量

230

审稿时长

>12 weeks

期刊介绍： Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes