Design of dual stimuli-responsive gels with physical and chemical properties that vary in response to light and temperature and cell behavior on their surfaces

IF 2.3 4区 化学 Q3 POLYMER SCIENCE Polymer Journal Pub Date : 2023-12-26 DOI:10.1038/s41428-023-00865-7
Masaaki Okihara, Akana Matsuda, Akifumi Kawamura, Takashi Miyata
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Abstract

Cell behaviors are highly sensitive to the surrounding environment. Therefore, in regulating cells, biomaterial substrates should be designed so their properties are similar to the surrounding environments of the cells. For cell regulation, we designed dual stimuli-responsive gels whose physical (elastic modulus) and chemical (hydrophilicity) properties can be changed by varying the UV exposure time and temperature, respectively. A dual stimuli-responsive polymer with photodimerizable groups and temperature-responsive moieties was prepared by copolymerizing 7-methacryloyloxycoumarin (MAC) and methoxyoligoethylene glycol methacrylate (OEGMA). The resulting polymers (P(MAC-co-OEGMA)) had lower critical solution temperatures (LCSTs), which depended on the compositions. A buffer solution containing P(MAC-co-OEGMA) was exposed to ultraviolet light (UV) for gelation, and the elastic modulus increased with increasing exposure time. The cell behavior, including adhesion and spreading, on the surfaces of these gels was investigated. Most of the cells adhered to P(MAC-co-OEGMA) gels with higher elastic moduli, and the cells were spread more effectively at temperatures above the LCST. This was because cell adhesion and spreading were strongly influenced by the physical and chemical properties of the P(MAC-co-OEGMA) gels, which were regulated by varying the UV exposure time and temperature. A buffer solution of dual stimuli-responsive polymer with photodimerizable groups and temperature-responsive moieties undergoes a phase transition from a sol state to a gel state by exposure to ultraviolet light. The resulting gels changed the physical (elastic modulus) and chemical (hydrophilicity) properties by varying UV exposure time and temperature, respectively. Cell adhesion, spreading, and proliferation were strongly influenced by the physical and chemical properties of the polymer gels, which were regulated by varying UV exposure time and temperature.

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设计具有物理和化学特性的双刺激响应凝胶,使其对光线和温度以及表面的细胞行为产生不同的响应
细胞行为对周围环境高度敏感。因此,在调控细胞时,生物材料基底的设计应使其特性与细胞周围环境相似。为了调节细胞,我们设计了双重刺激响应凝胶,通过改变紫外线照射时间和温度,可分别改变其物理(弹性模量)和化学(亲水性)特性。通过共聚 7-甲基丙烯酰氧基香豆素(MAC)和甲氧基环氧乙烷甲基丙烯酸酯(OEGMA),制备了一种具有光二聚化基团和温度响应分子的双重刺激响应聚合物。所制备的聚合物(P(MAC-co-OEGMA))具有较低的临界溶液温度(LCST),这取决于其成分。将含有 P(MAC-co-OEGMA)的缓冲溶液暴露在紫外线(UV)下进行凝胶化,弹性模量随着暴露时间的延长而增加。研究了细胞在这些凝胶表面的行为,包括粘附和扩散。大多数细胞都粘附在弹性模量较高的 P(MAC-co-OEGMA)凝胶上,而且细胞在 LCST 以上的温度下扩散得更有效。这是因为细胞的粘附和扩散受到 P(MAC-co-OEGMA)凝胶物理和化学特性的强烈影响,而这些特性可通过改变紫外线照射时间和温度来调节。
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来源期刊
Polymer Journal
Polymer Journal 化学-高分子科学
CiteScore
5.60
自引率
7.10%
发文量
131
审稿时长
2.5 months
期刊介绍: Polymer Journal promotes research from all aspects of polymer science from anywhere in the world and aims to provide an integrated platform for scientific communication that assists the advancement of polymer science and related fields. The journal publishes Original Articles, Notes, Short Communications and Reviews. Subject areas and topics of particular interest within the journal''s scope include, but are not limited to, those listed below: Polymer synthesis and reactions Polymer structures Physical properties of polymers Polymer surface and interfaces Functional polymers Supramolecular polymers Self-assembled materials Biopolymers and bio-related polymer materials Polymer engineering.
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