A green pathway for lignin valorization: Enzymatic lignin depolymerization in biocompatible ionic liquids and deep eutectic solvents

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Enzyme and Microbial Technology Pub Date : 2023-12-26 DOI:10.1016/j.enzmictec.2023.110392

Enshi Liu , Martha Inés Vélez Mercado , Fernando Segato , Mark R. Wilkins

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Abstract

Lignin depolymerization, which enables the breakdown of a complex and heterogeneous aromatic polymer into relatively uniform derivatives, serves as a critical process in valorization of lignin. Enzymatic lignin depolymerization has become a promising biological strategy to overcome the heterogeneity of lignin, due to its mild reaction conditions and high specificity. However, the low solubility of lignin compounds in aqueous environments prevents efficient lignin depolymerization by lignin-degrading enzymes. The employment of biocompatible ionic liquids (ILs) and deep eutectic solvents (DESs) in lignin fractionation has created a promising pathway to enzymatically depolymerize lignin within these green solvents to increase lignin solubility. In this review, recent research progress on enzymatic lignin depolymerization, particularly in a consolidated process involving ILs/DESs is summarized. In addition, the interactions between lignin-degrading enzymes and solvent systems are explored, and potential protein engineering methodology to improve the performance of lignin-degrading enzymes is discussed. Consolidation of enzymatic lignin depolymerization and biocompatible ILs/DESs paves a sustainable, efficient, and synergistic way to convert lignin into value-added products.

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木质素价值化的绿色途径：在生物相容性离子液体和深共晶溶剂中进行酶解木质素

木质素解聚可将复杂的异质芳香族聚合物分解为相对均匀的衍生物，是木质素价值化的关键过程。酶法木质素解聚因其反应条件温和、特异性强，已成为克服木质素异质性的一种前景广阔的生物策略。然而，木质素化合物在水环境中的低溶解度阻碍了木质素降解酶对木质素的有效解聚。在木质素分馏中使用生物相容性离子液体（ILs）和深共晶溶剂（DESs）为在这些绿色溶剂中酶解木质素以提高木质素溶解度开辟了一条前景广阔的途径。本综述总结了酶解木质素的最新研究进展，特别是涉及 ILs/DESs 的整合工艺。此外，还探讨了木质素降解酶与溶剂系统之间的相互作用，并讨论了提高木质素降解酶性能的潜在蛋白质工程方法。酶解木质素与生物相容性 ILs/DES 的结合为将木质素转化为高附加值产品铺平了一条可持续、高效和协同的道路。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.