Adaptation of Anadara kagoshimensis (Tokunaga, 1906) to Hypo- and Hyperosmotic Environment: Hemocyte Response

Pub Date : 2023-12-01 DOI:10.1134/s2075111723040094

{"title":"Adaptation of Anadara kagoshimensis (Tokunaga, 1906) to Hypo- and Hyperosmotic Environment: Hemocyte Response","authors":"","doi":"10.1134/s2075111723040094","DOIUrl":null,"url":null,"abstract":" <h3>Abstract</h3> Anadara kagoshimensis (Tokunaga, 1906) is an alien bivalve mollusk that has successfully colonized the Black Sea and the Sea of Azov despite a significantly lower salinity level of these waters compared to its native region. The morphological and morphometric characteristics of erythrocytes from A. kagoshimensis during adaptation to hypo- and hyperosmotic experimental conditions were analyzed by light microscopy. The control group of mollusks was maintained at 18‰ salinity. Experimental groups were maintained at the salinity levels of 8, 14, 35, and 45‰. A decreased salinity level was obtained by diluting seawater with distilled water at a rate of 1.5 ± 0.5‰ per day. An increased salinity was obtained by addition of sea salt to an aquarium at a rate of 2.5 ± 0.5‰ per day. The exposure period was 2 days. The natural salinity range of A. kagoshimensis was found to fall within 14—35‰. No significant cell morphology changes were observed under such conditions. At the same time, exposure of the mollusks to the environmental salinity of 8 and 45‰ caused an obvious stress expressed via appearance of cell anomalies and changes in the linear characteristics of erythrocytes. At the same time, no cell lysis was observed, and the values of the specific surface area and the nuclear-cytoplasmic ratio remained unchanged. The results of the study indicate the ability of the mollusk to survive for some time in an aquatic environment with extremely low or high salinity. ","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s2075111723040094","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Anadara kagoshimensis (Tokunaga, 1906) is an alien bivalve mollusk that has successfully colonized the Black Sea and the Sea of Azov despite a significantly lower salinity level of these waters compared to its native region. The morphological and morphometric characteristics of erythrocytes from A. kagoshimensis during adaptation to hypo- and hyperosmotic experimental conditions were analyzed by light microscopy. The control group of mollusks was maintained at 18‰ salinity. Experimental groups were maintained at the salinity levels of 8, 14, 35, and 45‰. A decreased salinity level was obtained by diluting seawater with distilled water at a rate of 1.5 ± 0.5‰ per day. An increased salinity was obtained by addition of sea salt to an aquarium at a rate of 2.5 ± 0.5‰ per day. The exposure period was 2 days. The natural salinity range of A. kagoshimensis was found to fall within 14—35‰. No significant cell morphology changes were observed under such conditions. At the same time, exposure of the mollusks to the environmental salinity of 8 and 45‰ caused an obvious stress expressed via appearance of cell anomalies and changes in the linear characteristics of erythrocytes. At the same time, no cell lysis was observed, and the values of the specific surface area and the nuclear-cytoplasmic ratio remained unchanged. The results of the study indicate the ability of the mollusk to survive for some time in an aquatic environment with extremely low or high salinity.

查看原文

微信好友朋友圈 QQ好友复制链接

Anadara kagoshimensis (Tokunaga, 1906) 对低渗透和高渗透环境的适应性：血细胞反应

摘要 Anadara kagoshimensis（Tokunaga，1906 年）是一种外来的双壳软体动物，尽管黑海和亚速海的盐度明显低于其原产地，但它还是成功地在这些水域定居下来。通过光学显微镜分析了 A. kagoshimensis 在适应低渗和高渗实验条件期间的红细胞形态和形态计量特征。对照组软体动物保持在 18‰的盐度下。实验组的盐度分别为 8、14、35 和 45‰。每天以 1.5 ± 0.5‰的速度用蒸馏水稀释海水，以降低盐度。每天以 2.5 ± 0.5‰的速度向水族箱中添加海盐，可提高盐度。暴露期为 2 天。发现 A. kagoshimensis 的自然盐度范围为 14-35‰。在这种条件下没有观察到明显的细胞形态变化。同时，将软体动物暴露在 8 和 45‰的环境盐度下会导致明显的应激反应，表现为细胞异常和红细胞线性特征的变化。与此同时，没有观察到细胞溶解现象，比表面积和核质比值保持不变。研究结果表明，软体动物有能力在盐度极低或极高的水生环境中存活一段时间。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助