Chronic stress-induced anxiety-like behavior, hippocampal oxidative, and endoplasmic reticulum stress are reversed by young plasma transfusion in aged adult rats.

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Iranian Journal of Basic Medical Sciences Pub Date : 2024-01-01 DOI:10.22038/IJBMS.2023.72437.15754
Arshad Ghaffari-Nasab, Gonja Javani, Fereshteh Farajdokht, Mohammad Reza Alipour, Gisou Mohaddes
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Abstract

Objectives: Aging and stress synergistically induce behavioral dysfunctions associated with oxidative and endoplasmic reticulum (ER) stress in brain regions. Considering the rejuvenating effects of young plasma on aging brain function, in the current study, we examined the effects of young plasma administration on anxiety-like behavior, NADH oxidase, NADPH oxidase, and ER stress markers in the hippocampus of old male rats.

Materials and methods: Young (3 months old) and aged (22 months old) rats were randomly assigned into five groups: young control (Y), aged control (A), aged rats subjected to chronic stress for four weeks (A+S), aged rats subjected to chronic stress and treated with old plasma (A+S+OP), and aged rats subjected to chronic stress and treated with young plasma (A+S+YP). Systemic injection of (1 ml) young and old plasma was performed for four weeks (3 times/week).

Results: Young plasma transfusion significantly improved anxiety-like behavior in aged rats and modulated oxidative stress in the hippocampus, evidenced by the increased NADH oxidase (NOX) activity and the reduced NADPH oxidase. In addition, the levels of C/EBP homologous protein (CHOP) and Glucose-Regulated Protein 78 (GRP-78), as ER stress markers, markedly reduced in the hippocampus following the administration of young plasma.

Conclusion: These findings suggest that young plasma transfusion could reverse anxiety-like behavior in stress-exposed aged rats by modulating the hippocampal oxidative and ER stress markers.

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输注年轻血浆可逆转老年成年大鼠由慢性应激诱发的焦虑样行为、海马氧化应激和内质网应激。
目的:衰老和应激会协同诱发与脑区氧化和内质网(ER)应激有关的行为功能障碍。考虑到年轻血浆对衰老脑功能的恢复作用,在本研究中,我们考察了年轻血浆给药对老年雄性大鼠海马焦虑样行为、NADH氧化酶、NADPH氧化酶和ER应激标记物的影响:将幼年(3 个月大)和老年(22 个月大)大鼠随机分为五组:幼年对照组(Y)、老年对照组(A)、接受四周慢性应激的老年大鼠(A+S)、接受慢性应激并用老年血浆治疗的老年大鼠(A+S+OP)和接受慢性应激并用年轻血浆治疗的老年大鼠(A+S+YP)。大鼠全身注射(1 毫升)年轻血浆和陈旧血浆,连续四周(每周 3 次):结果:输注年轻血浆能明显改善老年大鼠的焦虑样行为,并调节海马的氧化应激,表现为 NADH 氧化酶(NOX)活性增加和 NADPH 氧化酶减少。此外,作为ER应激标志物的C/EBP同源蛋白(CHOP)和葡萄糖调节蛋白78(GRP-78)的水平在给予年轻血浆后在海马中明显降低:这些研究结果表明,输注年轻血浆可通过调节海马氧化和ER应激标记物来逆转应激暴露老年大鼠的焦虑样行为。
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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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