{"title":"Antioxidant and antiproliferative effects of <i>Teucrium polium</i> extract: computational and <i>in vivo</i> study in rats.","authors":"Fatma Rahmouni, Latifa Hamdaoui, Mongi Saoudi, Riadh Badraoui, Tarek Rebai","doi":"10.1080/15376516.2023.2301670","DOIUrl":null,"url":null,"abstract":"<p><p>The current study aimed to assess the antioxidant and antiproliferative effects of <i>teucrium polium</i> extract: computational and <i>in vivo</i> study in rats. Three groups of animals: Group (i) constitute the control group; Group (ii) HeLa group received an intrafemoral inoculation of HeLa cells and Group (iii) constitue the combination between HeLa + <i>T. polium</i>. The plant was administered by gavage. Our results revealed that HeLa cell injection showed an elevation in aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), creatinine, urea, calcium and phosphorus. The pretreatment with the plant extract reduced the level of these parameters. Injection of HeLa cells showed a significant decrease in phosphorus and calcium respectively. However, the pretreatment by <i>T. polium</i> modulated the level of these two minerals. Rats treated with HeLa cells line showed an increase in the level of lipid peroxidation as evaluated by the TBARS substances, at the same time, a significant decreases in SOD, CAT and GPx activities were noted in the HeLa group compared to the control. On the other hand, pretreatment with the plant improved the level of these enzymes. Our results revealed that <i>T.polium</i> has a therapeutic effect on some health problems. HeLa cell line induced a small infiltration in liver and kidney. <i>T. polium</i> reduced the damage in both liver and kidney, but did not reveal any proliferation of tumor cells from trabecular bone tissue. The computational study revealed that <i>T. polium</i> compound bound with high free binding energies and established promising network of molecular interactions with COX-2 and TNF-α macromolecules.</p>","PeriodicalId":23177,"journal":{"name":"Toxicology Mechanisms and Methods","volume":" ","pages":"495-506"},"PeriodicalIF":3.2000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicology Mechanisms and Methods","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/15376516.2023.2301670","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/26 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
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Abstract
The current study aimed to assess the antioxidant and antiproliferative effects of teucrium polium extract: computational and in vivo study in rats. Three groups of animals: Group (i) constitute the control group; Group (ii) HeLa group received an intrafemoral inoculation of HeLa cells and Group (iii) constitue the combination between HeLa + T. polium. The plant was administered by gavage. Our results revealed that HeLa cell injection showed an elevation in aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), creatinine, urea, calcium and phosphorus. The pretreatment with the plant extract reduced the level of these parameters. Injection of HeLa cells showed a significant decrease in phosphorus and calcium respectively. However, the pretreatment by T. polium modulated the level of these two minerals. Rats treated with HeLa cells line showed an increase in the level of lipid peroxidation as evaluated by the TBARS substances, at the same time, a significant decreases in SOD, CAT and GPx activities were noted in the HeLa group compared to the control. On the other hand, pretreatment with the plant improved the level of these enzymes. Our results revealed that T.polium has a therapeutic effect on some health problems. HeLa cell line induced a small infiltration in liver and kidney. T. polium reduced the damage in both liver and kidney, but did not reveal any proliferation of tumor cells from trabecular bone tissue. The computational study revealed that T. polium compound bound with high free binding energies and established promising network of molecular interactions with COX-2 and TNF-α macromolecules.
本研究旨在评估柚木提取物的抗氧化和抗增殖作用：大鼠计算和体内研究。动物分为三组：(i)组为对照组；(ii) HeLa 组接受 HeLa 细胞股内接种；(iii) HeLa + T. polium 组。植物以灌胃方式给药。结果显示，注射 HeLa 细胞后，天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、总胆红素（TB）、肌酐、尿素、钙和磷均升高。植物提取物的预处理降低了这些参数的水平。注射 HeLa 细胞后，磷和钙的含量分别显著下降，但 T. polium 的预处理调节了这两种矿物质的含量。用 HeLa 细胞系处理的大鼠的脂质过氧化水平（用 TBARS 物质评估）有所增加，同时，与对照组相比，HeLa 组的 SOD、CAT 和 GPx 活性明显降低。另一方面，使用该植物进行预处理可提高这些酶的水平。我们的研究结果表明，T.polium 对一些健康问题有治疗作用。HeLa 细胞系在肝脏和肾脏中诱发了少量浸润。枸杞多糖减轻了肝脏和肾脏的损伤，但没有发现骨小梁组织中有肿瘤细胞增殖。计算研究显示，枸杞多糖化合物与 COX-2 和 TNF-α 大分子的自由结合能很高，并建立了良好的分子相互作用网络。
期刊介绍：
Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy.
Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including:
In vivo studies with standard and alternative species
In vitro studies and alternative methodologies
Molecular, biochemical, and cellular techniques
Pharmacokinetics and pharmacodynamics
Mathematical modeling and computer programs
Forensic analyses
Risk assessment
Data collection and analysis.
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