Potential induction of hyperkeratosis in rats' cervi by gentamicin via induction of oxidative stress, inflammation and apoptosis.

Human & experimental toxicology Pub Date : 2024-01-01 DOI:10.1177/09603271231225744

Walaa Yehia Abdelzaher, Mina Ezzat Attya, Mohamed Ahmed Zeen El-Din, Alaa Gamal El Satae, Hatem A Mohamed, Remon R Rofaeil

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Abstract

The present study aimed to identify the possible effect of gentamicin (GEN) in Rats' Cervi. Estradiol Valerate (EV) was used to induce cervical hyperkeratosis. GEN was administered in absence of EV. Serum and cervical GEN concentration were determined. Levels of malondialdehyde (MDA), total nitrites/nitrate (NOx), reduced glutathione (GSH), tumor necrosis factor-α (TNF-α), sirtuin type 1 (Sirt1) and nuclear factor (erythroid-derived 2)-like-2 factors (Nrf2) were measured in cervix tissue. Expression of BAX and Bcl2 were determined. Cervical histopathological examination was done. EV and GEN significantly increased MDA, NOx, TNF-α and BAX/Bcl2 ratio with decrease in GSH, Nrf2 and Sirt1 levels in cervical tissue. Histopathological picture of diffuse and marked hyperkeratosis was detected in EV and GEN groups. In conclusion, GEN-induced cervical hyperkeratosis via induction of oxidative stress, inflammation and apoptosis.

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庆大霉素通过诱导氧化应激、炎症和细胞凋亡可能诱导大鼠宫颈角化过度。

本研究旨在确定庆大霉素（GEN）对大鼠宫颈可能产生的影响。戊酸雌二醇（EV）用于诱导宫颈角化过度。在没有使用 EV 的情况下使用 GEN。测定血清和宫颈的 GEN 浓度。测量宫颈组织中丙二醛（MDA）、总亚硝酸盐/硝酸盐（NOx）、还原型谷胱甘肽（GSH）、肿瘤坏死因子-α（TNF-α）、sirtuin 1 型（Sirt1）和核因子（红细胞衍生 2）-类-2 因子（Nrf2）的水平。还测定了 BAX 和 Bcl2 的表达。进行了宫颈组织病理学检查。EV 和 GEN 能明显增加宫颈组织中的 MDA、NOx、TNF-α 和 BAX/Bcl2 比率，同时降低 GSH、Nrf2 和 Sirt1 水平。在 EV 组和 GEN 组中发现了弥漫性和明显的角化过度的组织病理学图像。总之，GEN 通过诱导氧化应激、炎症和细胞凋亡诱导宫颈过度角化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Human & experimental toxicology

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